ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1)

NMDA1; NMDAR1; NR1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1

Specificity

This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1).
No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Malaysian Journal of Medicine and Health Sciences Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring
ВЛИЯНИЕ ХРОНИЧЕСКОГО ВВЕДЕНИЯ НЕЙРОЛЕПТИКОВ НА УРОВНИ НЕЙРОСПЕЦИФИЧЕСКИХ АУТОАНТИТЕЛ В КРОВИ У КРЫС
Turk J Pharm Sci Tualang Honey Improves Memory and Prevents Hippocampal Changes in Prenatally Stressed Rats 33389951
J Trace Elem Med Biol The Association between Blood Lymphocyte NMDAR, group I mGluRs and Cognitive Function Changes in Occupationally Aluminum-exposed Workers and … 34673477
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPE805Ra01 Recombinant Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) Positive Control; Immunogen; SDS-PAGE; WB.
PAE805Ra01 Polyclonal Antibody to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) WB; IHC; ICC; IP.
MAE805Ra21 Monoclonal Antibody to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) WB; IHC; ICC; IP.
SEE805Ra ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) Enzyme-linked immunosorbent assay for Antigen Detection.
LME805Ra Multiplex Assay Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.