ELISA Kit for Tubulin Beta 1 (TUBb1)

Specificity

This assay has high sensitivity and excellent specificity for detection of Tubulin Beta 1 (TUBb1).
No significant cross-reactivity or interference between Tubulin Beta 1 (TUBb1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tubulin Beta 1 (TUBb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tubulin Beta 1 (TUBb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Free Radic Biol Med. DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts. Pubmed:26806551
MOLECULES New Paracyclophanylthiazoles with Anti-Leukemia Activity: Design, Synthesis, Molecular Docking, and Mechanistic Studies Pubmed: 32645912
Russian Journal of Bioorganic Chemistry volume Facile Synthesis of New Hybrid 2-Quinlolinone Derivatives Structures as Anticancer Drugs for Breast Cancer Treatment
Biochemistry (Moscow) VPg of Potato Virus Y and Potato Cap-Binding eIF4E Factors: Selective Interaction and Its Supposed Mechanism 34565316
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPE707Mu01 Recombinant Tubulin Beta 1 (TUBb1) Positive Control; Immunogen; SDS-PAGE; WB.
RPE707Mu02 Recombinant Tubulin Beta 1 (TUBb1) Positive Control; Immunogen; SDS-PAGE; WB.
PAE707Mu01 Polyclonal Antibody to Tubulin Beta 1 (TUBb1) WB; IHC; ICC; IP.
PAE707Mu02 Polyclonal Antibody to Tubulin Beta 1 (TUBb1) WB; IHC; ICC; IP.
MAE707Mu21 Monoclonal Antibody to Tubulin Beta 1 (TUBb1) WB; IHC; ICC; IP.
SEE707Mu ELISA Kit for Tubulin Beta 1 (TUBb1) Enzyme-linked immunosorbent assay for Antigen Detection.
LME707Mu Multiplex Assay Kit for Tubulin Beta 1 (TUBb1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.