ELISA Kit for S100 Calcium Binding Protein A7 (S100A7)

S100-A7; PSOR1; S100A7c; Psoriasin 1

Specificity

This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A7 (S100A7).
No significant cross-reactivity or interference between S100 Calcium Binding Protein A7 (S100A7) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant S100 Calcium Binding Protein A7 (S100A7) and the recovery rates were calculated by comparing the measured value to the expected amount of S100 Calcium Binding Protein A7 (S100A7) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 95
EDTA plasma(n=5) 84-98 89
heparin plasma(n=5) 80-91 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A7 (S100A7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A7 (S100A7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100 Calcium Binding Protein A7 (S100A7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-99% 82-101% 89-96% 85-92%
EDTA plasma(n=5) 90-97% 93-105% 88-95% 91-101%
heparin plasma(n=5) 88-96% 92-101% 88-103% 91-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Archives of Dermatological Research Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid esters SpringerLink: t07u24t43r357l15
J Proteome Res Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS Approach PubMed: 25807139
Exp Dermatol S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signaling Pubmed:27060579
Experimental Dermatology S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL‐6 secretion through IκB/NF‐κB signalling pubmed:27060579
Experiental And Therapeutic Medicine Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription pubmed:28672924
Scientific Reports The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function. pubmed:29142248
Journal of the European Academy of Dermatology and Venereology A potential contribution of psoriasin to vascular and epithelial abnormalities and inflammation in systemic sclerosis Pubmed:28681537
Scientific Reports Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique Pubmed:29374283
Neuroscience Bulletin Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state Pubmed:30019218
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945
Mediators of Inflammation Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris
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