ELISA Kit for Apolipoprotein A4 (APOA4)

ApoA-IV; ApoAIV; Apo-A4


This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A4 (APOA4).
No significant cross-reactivity or interference between Apolipoprotein A4 (APOA4) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Apolipoprotein A4 (APOA4) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein A4 (APOA4) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-104 96
EDTA plasma(n=5) 78-99 86
heparin plasma(n=5) 91-98 95


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein A4 (APOA4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-93% 83-96% 84-103% 98-105%
EDTA plasma(n=5) 95-104% 80-96% 80-101% 90-97%
heparin plasma(n=5) 95-103% 80-101% 89-103% 90-104%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Biochimica et Biophysica Acta Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties PubMed: 23328279
Exp Ther Med. Differential plasma proteome analysis in patients with high?altitude pulmonary edema at the acute and recovery phases Pubmed:24940404
EuPA Open Proteomics Human maternal plasma proteomic changes with parturition Sciencedirect:S2212968514000506
BJOG Development of concise audit standards for unplanned return to theate following gynaecological surgery at Gloucestershire Royal Hospital Doi: 10.1111
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