ELISA Kit for Apolipoprotein A4 (APOA4)
ApoA-IV; ApoAIV; Apo-A4
- Product No.SEB967Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range6.25-400ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.62ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 798
For more details, please contact local distributors! US$ 3591
For more details, please contact local distributors! US$ 6783
For more details, please contact local distributors! US$ 55860
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A4 (APOA4).
No significant cross-reactivity or interference between Apolipoprotein A4 (APOA4) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Apolipoprotein A4 (APOA4) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein A4 (APOA4) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein A4 (APOA4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Biochimica et Biophysica Acta||Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties PubMed: 23328279|
|Exp Ther Med.||Differential plasma proteome analysis in patients with high?altitude pulmonary edema at the acute and recovery phases Pubmed:24940404|
|EuPA Open Proteomics||Human maternal plasma proteomic changes with parturition Sciencedirect:S2212968514000506|
|BJOG||Development of concise audit standards for unplanned return to theate following gynaecological surgery at Gloucestershire Royal Hospital Doi: 10.1111|
|Catalog No.||Related products for research use of Sus scrofa; Porcine (Pig) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB967Po01||Recombinant Apolipoprotein A4 (APOA4)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB967Po01||Polyclonal Antibody to Apolipoprotein A4 (APOA4)||WB; IHC; ICC; IP.|
|LAB967Po81||FITC-Linked Polyclonal Antibody to Apolipoprotein A4 (APOA4)||WB; IHC; ICC; IF.|
|LAB967Po71||Biotin-Linked Polyclonal Antibody to Apolipoprotein A4 (APOA4)||WB; IHC; ICC.|
|MAB967Po21||Monoclonal Antibody to Apolipoprotein A4 (APOA4)||WB; IHC; ICC; IP.|
|LAB967Po72||Biotin-Linked Monoclonal Antibody to Apolipoprotein A4 (APOA4)||WB; IHC; ICC.|
|SEB967Po||ELISA Kit for Apolipoprotein A4 (APOA4)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PSB967Po01||Antibody Pair for Apolipoprotein A4 (APOA4)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSB967Po01||ELISA Kit DIY Materials for Apolipoprotein A4 (APOA4)||Main materials for "Do It(ELISA Kit) Yourself"|