ELISA Kit for Apolipoprotein C3 (APOC3)
Apo-C3; APOCIII; APOC-III
- Product No.SEB890Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range93.75-6,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 37.59pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Apolipoprotein C3 (APOC3).
No significant cross-reactivity or interference between Apolipoprotein C3 (APOC3) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Apolipoprotein C3 (APOC3) and the recovery rates were calculated by comparing the measured value to the expected amount of Apolipoprotein C3 (APOC3) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein C3 (APOC3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein C3 (APOC3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apolipoprotein C3 (APOC3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Atherosclerosis||Up-reCavia (Guinea pig )lation of Hnf1α gene expression in the liver of rats with experimentally induced chronic renal failure – A possible link between circulating PCSK9 and triacylglycerol concentrations Pubmed:26978583|
|The Journal of Lipid Research||Effects of angiopoietin-like protein 3 deficiency on postprandial lipid and lipoprotein metabolism Pubmed:27040449|
|Journal of Chromatography A||Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis S0021967317311858|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|APB890Hu01||Active Apolipoprotein C3 (APOC3)||Cell culture; Activity Assays.|
|RPB890Hu01||Recombinant Apolipoprotein C3 (APOC3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB890Hu01||Polyclonal Antibody to Apolipoprotein C3 (APOC3)||WB; ICC; IHC-P; IHC-F; ELISA.|
|MAB890Hu22||Monoclonal Antibody to Apolipoprotein C3 (APOC3)||WB; IHC; ICC; IP.|
|SEB890Hu||ELISA Kit for Apolipoprotein C3 (APOC3)||Enzyme-linked immunosorbent assay for Antigen Detection.|