ELISA Kit for Alkaline Phosphatase (ALP)
AKP; ALKP; Basic Phosphatase
- Product No.SEB472Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.25-80ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.53ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 722
For more details, please contact local distributors! US$ 3249
For more details, please contact local distributors! US$ 6137
For more details, please contact local distributors! US$ 50540
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase (ALP).
No significant cross-reactivity or interference between Alkaline Phosphatase (ALP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Alkaline Phosphatase (ALP) and the recovery rates were calculated by comparing the measured value to the expected amount of Alkaline Phosphatase (ALP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alkaline Phosphatase (ALP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Clinical Oral Implants Research||Comparison of platelet rich fibrin and collagen as osteoblast-seeded scaffolds for bone tissue engineering applications Wiley: source|
|Die Pharmazie - An International Journal of Pharmaceutical Sciences||The role of atorvastatin in bone metabolism in male albino Wistar rats Ingenta: art00010|
|22||Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses ScienceDirect: S0142961214003135|
|Biomed Mater.?||Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering Pubmed:24770944|
|Journal of Colloid and Interface Science||Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses Pubmed:25706198|
|Bone||The active role of osteoporosis in the interaction between osteoblasts and bone metastases PubMed: 26057367|
|Macromolecular Bioscience||Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells PubMed: 26126665|
|journal of hard Tissue Biology||Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite Article: Jhtb|
|Colloids Surf B Biointerfaces||Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation PubMed: 26445021|
|Gels||On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery 2310-2861: 1|
|Acta Biomater||Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study PubMed: 26689470|
|Sci Rep||A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration PubMed: 26632447|
|Acta Biomaterialia||Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study Pubmed:26689470|
|PLoS One.||Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. pubmed:28158228|
|Scientific Reports||Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302|
|J Cell Physiol.||An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. pubmed:28240358|
|Biomedicine & Pharmacotherapy||Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats S0753332216322752|
|Journal of Inorganic Biochemistry||Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. pubmed:29049953|
|Biomedicine & pharmacotherapy||Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|PAB472Ra01||Polyclonal Antibody to Alkaline Phosphatase (ALP)||WB; IHC; ICC; IP.|
|SEB472Ra||ELISA Kit for Alkaline Phosphatase (ALP)||Enzyme-linked immunosorbent assay for Antigen Detection.|