Polyclonal Antibody to Alkaline Phosphatase (ALP)

AKP; ALKP; Basic Phosphatase

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ALP. It has been selected for its ability to recognize ALP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Clinical Oral Implants Research Comparison of platelet rich fibrin and collagen as osteoblast-seeded scaffolds for bone tissue engineering applications Wiley: source
Die Pharmazie - An International Journal of Pharmaceutical Sciences The role of atorvastatin in bone metabolism in male albino Wistar rats Ingenta: art00010
22 Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses ScienceDirect: S0142961214003135
Biomed Mater.? Directing chondrogenic differentiation of mesenchymal stem cells with a solid-supported chitosan thermogel for cartilage tissue engineering Pubmed:24770944
Journal of Colloid and Interface Science Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses Pubmed:25706198
Bone The active role of osteoporosis in the interaction between osteoblasts and bone metastases PubMed: 26057367
Macromolecular Bioscience Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells PubMed: 26126665
journal of hard Tissue Biology Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite Article: Jhtb
Colloids Surf B Biointerfaces Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation PubMed: 26445021
Gels On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery 2310-2861: 1
Acta Biomater Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study PubMed: 26689470
Sci Rep A composite scaffold of MSC affinity peptide-modified demineralized bone matrix particles and chitosan hydrogel for cartilage regeneration PubMed: 26632447
Acta Biomaterialia Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study Pubmed:26689470
PLoS One. Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. pubmed:28158228
Scientific Reports Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302
J Cell Physiol. An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. pubmed:28240358
Biomedicine & Pharmacotherapy Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats S0753332216322752
Journal of Inorganic Biochemistry Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. pubmed:29049953
Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
PAB472Ra01 Polyclonal Antibody to Alkaline Phosphatase (ALP) WB; IHC; ICC; IP.
SEB472Ra ELISA Kit for Alkaline Phosphatase (ALP) Enzyme-linked immunosorbent assay for Antigen Detection.