ELISA Kit for Lipopolysaccharide Binding Protein (LBP)

LPS-Binding Protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Lipopolysaccharide Binding Protein (LBP).
No significant cross-reactivity or interference between Lipopolysaccharide Binding Protein (LBP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Lipopolysaccharide Binding Protein (LBP) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipopolysaccharide Binding Protein (LBP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-104 94
EDTA plasma(n=5) 88-103 93
heparin plasma(n=5) 85-94 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipopolysaccharide Binding Protein (LBP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 84-91% 83-101% 90-98%
EDTA plasma(n=5) 78-99% 80-93% 95-102% 79-93%
heparin plasma(n=5) 81-95% 92-101% 83-97% 83-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
37 A Marker of Endotoxemia Is Associated With Obesity and Related Metabolic Disorders in Apparently Healthy Chinese Diabetes: source
AIDS Oral serum-derived bovine immunoglobulin improves duodenal immune reconstitution and absorption function in patients with HIV enteropathy PubMed: PMC3754419
31 Gut microbiome composition is linked to whole grain-induced immunological improvements Pubmed: 23038174
The Veterinary Journal High ambient temperature alleviates the inflammatory response and growth depression in pigs challenged with Escherichia coli lipopolysaccharide Pubmed: 24792207
Perfusion The effect of normovolemic modified ultrafiltration on inflammatory mediators, endotoxins, terminal complement complexes and clinical outcome in high-risk cardiac surgery patients Pubmed: 23429100
FEMS Microbiology Ecology A gut microbiota-targeted dietary intervention for amelioration of chronic inflammation underlying metabolic syndrome. Pubmed: 24117923
Digestive diseases and sciences Frequency and Severity of Cirrhotic Cardiomyopathy and Its Possible Relationship with Bacterial Endotoxemia Pubmed: 23907333
Atherosclerosis Circulating pathogen associated molecular pattern – Binding proteins and High Mobility Group Box protein 1 in nascent metabolic syndrome: Implications for cellular Toll-like receptor activity Pubmed:25063948
BMC Complementary and Alternative Medicine Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121
Atherosclerosis Increased adipose tissue secretion of Fetuin-A, lipopolysaccharide-binding protein and high-mobility group box protein 1 in metabolic syndrome PubMed: 25978344
Annals of Gastroenterology Systemic levels of human β-defensin 1 are elevated in patients with cirrhosis PubMed: 26751578
Food Chem. Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk Pubmed:27173528
Liver International High serum lipopolysaccharide binding protein is associated with increased mortality in patients with decompensated cirrhosis pubmed:27712029
Journal of Functional Foods Modulation of gut microbiota and gut-generated metabolites by bitter melon results in improvement in the metabolic status in high fat diet-induced obese rats 10.1016/j.jff.2017.12.050
Scientific Reports Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains. pubmed:28512356
Journal of ethnopharmacology A standardized extract of the fruit of Hovenia dulcis alleviated alcohol-induced hangover in healthy subjects with heterozygous ALDH2: A randomized, controlled, crossover trial. pubmed:28750942
Journal of Allergy and Clinical Immunology On a Western diet, APOE ɛ4 is associated with low innate immune sensing, but not APOE ɛ3 Pubmed:29928926
Journal of Animal Sciences Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations Pubmed:30060086
Journal of Animal Science Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood … Pubmed: 30184125
BMC Complementary and Alternative Medicine Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic … Pubmed: 30453950
Cell Communication and Signaling Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways
Nutrients High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
Journal of Animal Science Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers Pubmed: 31410465
Nutrients Lactobacillus reuteri V3401 Reduces Inflammatory Biomarkers and Modifies the Gastrointestinal Microbiome in Adults with Metabolic Syndrome: The PROSIR Study Pubmed: 31370223
journal of veterinary pharmacology and therapeutics Effects of sinomenine in LPS‐associated diseases are related to inhibition of LBP, Mac‐1, and L‐selectin levels Pubmed: 31490576
Amyloid Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein Pubmed: 31615282
Food Research International Ethanol Extract of Propolis Prevents High-Fat Diet-Induced Insulin Resistance and Obesity in Association with Modulation of Gut Microbiota in Mice Pubmed: 32156386
Journal of Translational Medicine Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation Pubmed: 33036625
Communications Biology Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in mice Pubmed: 32879433
MOLECULAR NUTRITION & FOOD RESEARCH Antibiotic Treatment Reduces the Health Benefits of Soy Protein Pubmed: 32729948
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity Pubmed: 32633953
fish & shellfish immunology Molecular characterization and expression analysis of chemokine (CXCL12) from Nile tilapia (Oreochromis niloticus) Pubmed: 32540504
Scientific Reports Tandem Mass Tag (TMT)-based quantitative proteomics reveals potential targets associated with onset of Sub-clinical Mastitis in cows Pubmed: 32518370
experimental eye research Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis Pubmed: 32376471
Frontiers in Microbiology Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPB406Bo01 Recombinant Lipopolysaccharide Binding Protein (LBP) Positive Control; Immunogen; SDS-PAGE; WB.
PAB406Bo01 Polyclonal Antibody to Lipopolysaccharide Binding Protein (LBP) WB; IHC; ICC; IP.
LAB406Bo71 Biotin-Linked Polyclonal Antibody to Lipopolysaccharide Binding Protein (LBP) WB; IHC; ICC.
MAB406Bo21 Monoclonal Antibody to Lipopolysaccharide Binding Protein (LBP) WB; IHC; ICC; IP.
SEB406Bo ELISA Kit for Lipopolysaccharide Binding Protein (LBP) Enzyme-linked immunosorbent assay for Antigen Detection.