ELISA Kit for Lipopolysaccharide Binding Protein (LBP)
- Product No.SEB406Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.625-40ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.291ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 798
For more details, please contact local distributors! US$ 3591
For more details, please contact local distributors! US$ 6783
For more details, please contact local distributors! US$ 55860
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Lipopolysaccharide Binding Protein (LBP).
No significant cross-reactivity or interference between Lipopolysaccharide Binding Protein (LBP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Lipopolysaccharide Binding Protein (LBP) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipopolysaccharide Binding Protein (LBP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipopolysaccharide Binding Protein (LBP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|37||A Marker of Endotoxemia Is Associated With Obesity and Related Metabolic Disorders in Apparently Healthy Chinese Diabetes: source|
|AIDS||Oral serum-derived bovine immunoglobulin improves duodenal immune reconstitution and absorption function in patients with HIV enteropathy PubMed: PMC3754419|
|31||Gut microbiome composition is linked to whole grain-induced immunological improvements Pubmed: 23038174|
|The Veterinary Journal||High ambient temperature alleviates the inflammatory response and growth depression in pigs challenged with Escherichia coli lipopolysaccharide Pubmed: 24792207|
|Perfusion||The effect of normovolemic modified ultrafiltration on inflammatory mediators, endotoxins, terminal complement complexes and clinical outcome in high-risk cardiac surgery patients Pubmed: 23429100|
|FEMS Microbiology Ecology||A gut microbiota-targeted dietary intervention for amelioration of chronic inflammation underlying metabolic syndrome. Pubmed: 24117923|
|Digestive diseases and sciences||Frequency and Severity of Cirrhotic Cardiomyopathy and Its Possible Relationship with Bacterial Endotoxemia Pubmed: 23907333|
|Atherosclerosis||Circulating pathogen associated molecular pattern – Binding proteins and High Mobility Group Box protein 1 in nascent metabolic syndrome: Implications for cellular Toll-like receptor activity Pubmed:25063948|
|BMC Complementary and Alternative Medicine||Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121|
|Atherosclerosis||Increased adipose tissue secretion of Fetuin-A, lipopolysaccharide-binding protein and high-mobility group box protein 1 in metabolic syndrome PubMed: 25978344|
|Annals of Gastroenterology||Systemic levels of human β-defensin 1 are elevated in patients with cirrhosis PubMed: 26751578|
|Food Chem.||Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk Pubmed:27173528|
|Liver International||High serum lipopolysaccharide binding protein is associated with increased mortality in patients with decompensated cirrhosis pubmed:27712029|
|Journal of Functional Foods||Modulation of gut microbiota and gut-generated metabolites by bitter melon results in improvement in the metabolic status in high fat diet-induced obese rats 10.1016/j.jff.2017.12.050|
|Scientific Reports||Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains. pubmed:28512356|
|Journal of ethnopharmacology||A standardized extract of the fruit of Hovenia dulcis alleviated alcohol-induced hangover in healthy subjects with heterozygous ALDH2: A randomized, controlled, crossover trial. pubmed:28750942|
|Journal of Allergy and Clinical Immunology||On a Western diet, APOE ɛ4 is associated with low innate immune sensing, but not APOE ɛ3 Pubmed:29928926|
|Journal of Animal Sciences||Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations Pubmed:30060086|
|Journal of Animal Science||Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood … Pubmed: 30184125|
|BMC Complementary and Alternative Medicine||Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic … Pubmed: 30453950|
|Cell Communication and Signaling||Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways|
|Nutrients||High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …|
|Journal of Animal Science||Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers Pubmed: 31410465|
|Nutrients||Lactobacillus reuteri V3401 Reduces Inflammatory Biomarkers and Modifies the Gastrointestinal Microbiome in Adults with Metabolic Syndrome: The PROSIR Study Pubmed: 31370223|
|journal of veterinary pharmacology and therapeutics||Effects of sinomenine in LPS‐associated diseases are related to inhibition of LBP, Mac‐1, and L‐selectin levels Pubmed: 31490576|
|Amyloid||Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein Pubmed: 31615282|
|Food Research International||Ethanol Extract of Propolis Prevents High-Fat Diet-Induced Insulin Resistance and Obesity in Association with Modulation of Gut Microbiota in Mice Pubmed: 32156386|
|Journal of Translational Medicine||Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation Pubmed: 33036625|
|Communications Biology||Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in mice Pubmed: 32879433|
|MOLECULAR NUTRITION & FOOD RESEARCH||Antibiotic Treatment Reduces the Health Benefits of Soy Protein Pubmed: 32729948|
|JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY||Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity Pubmed: 32633953|
|fish & shellfish immunology||Molecular characterization and expression analysis of chemokine (CXCL12) from Nile tilapia (Oreochromis niloticus) Pubmed: 32540504|
|Scientific Reports||Tandem Mass Tag (TMT)-based quantitative proteomics reveals potential targets associated with onset of Sub-clinical Mastitis in cows Pubmed: 32518370|
|experimental eye research||Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis Pubmed: 32376471|
|Frontiers in Microbiology||Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice|
|Catalog No.||Related products for research use of Bos taurus; Bovine (Cattle) Organism species||Applications (RESEARCH USE ONLY!)|
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