ELISA Kit for Agouti Related Protein (AGRP)

AGRT; ASIP2; ART; Agouti-Related Peptide

Specificity

This assay has high sensitivity and excellent specificity for detection of Agouti Related Protein (AGRP).
No significant cross-reactivity or interference between Agouti Related Protein (AGRP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Agouti Related Protein (AGRP) and the recovery rates were calculated by comparing the measured value to the expected amount of Agouti Related Protein (AGRP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-102 99
EDTA plasma(n=5) 84-98 87
heparin plasma(n=5) 78-103 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Agouti Related Protein (AGRP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Agouti Related Protein (AGRP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Agouti Related Protein (AGRP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-102% 85-101% 93-101% 81-105%
EDTA plasma(n=5) 79-95% 84-99% 97-104% 92-104%
heparin plasma(n=5) 87-97% 79-89% 86-101% 83-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Проблеми еколог МЕХАН Pdf: Mag3
Світ медицини та біології. ВИЗНАЧЕННЯ ВЗАЄМОЗВ’ЯЗКІВ ФАКТОРІВ ФОРМУВАННЯ ПІДВИЩЕНОЇ МАСИ ТІЛА У МОЛОДИХ ОСІБ SMB-2016-01-103.pdf
J Endocrinol Invest Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … Pubmed:35098494
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB302Mu01 Recombinant Agouti Related Protein (AGRP) Positive Control; Immunogen; SDS-PAGE; WB.
RPB302Mu02 Recombinant Agouti Related Protein (AGRP) Positive Control; Immunogen; SDS-PAGE; WB.
PAB302Mu02 Polyclonal Antibody to Agouti Related Protein (AGRP) WB; IHC; ICC; IP.
PAB302Mu01 Polyclonal Antibody to Agouti Related Protein (AGRP) WB; IHC; ICC; IP.
LAB302Mu71 Biotin-Linked Polyclonal Antibody to Agouti Related Protein (AGRP) WB; IHC; ICC.
SEB302Mu ELISA Kit for Agouti Related Protein (AGRP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB302Mu Multiplex Assay Kit for Agouti Related Protein (AGRP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB302Mu01 ELISA Kit DIY Materials for Agouti Related Protein (AGRP) Main materials for "Do It(ELISA Kit) Yourself"