ELISA Kit for Aspartate Aminotransferase (AST)

cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble

Specificity

This assay has high sensitivity and excellent specificity for detection of Aspartate Aminotransferase (AST).
No significant cross-reactivity or interference between Aspartate Aminotransferase (AST) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Aspartate Aminotransferase (AST) and the recovery rates were calculated by comparing the measured value to the expected amount of Aspartate Aminotransferase (AST) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-97 92
EDTA plasma(n=5) 84-92 88
heparin plasma(n=5) 80-101 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aspartate Aminotransferase (AST) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-98% 93-101% 79-89% 78-91%
EDTA plasma(n=5) 78-104% 94-102% 83-96% 78-97%
heparin plasma(n=5) 78-93% 92-101% 79-92% 84-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms PubMed: PMC3612362
Proceedings of the National Academy of Sciences Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse model PubMed: PMC3382536
African Journal of Pharmacy and Pharmacology Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats Academicjournals: Source
Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms Pubmed: 23325074
European Journal of Pharmacology Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats ScienceDirect: S0014299914000235
Journal of Immunology The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice Pubmed:25480563
Gastroenterol Res Pract Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning PubMed: 26697061
Gastroenterol Res Pract. Advanced Studies in Clinical and Experimental Research in Gastroenterology Pubmed:26955389
Scientific Reports Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302
 Biochimica et Biophysica Acta (BBA)-General Subjects Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats pubmed:28341485
Scientific Reports Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency Pubmed:29379059
FEBS Open Bio A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosis Pubmed:29321963
BMC Complementary and Alternative Medicine  Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents Pubmed: 28095910
laboratory investigation Hepatic pathology and altered gene transcription in a murine model of acid ceramidase deficiency Pubmed: 31186526
Brain Behavior and Immunity Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy Pubmed: 31437533
Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
SEB214Rb ELISA Kit for Aspartate Aminotransferase (AST) Enzyme-linked immunosorbent assay for Antigen Detection.