ELISA Kit for Endoglin (ENG)

CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Endoglin (ENG).
No significant cross-reactivity or interference between Endoglin (ENG) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Endoglin (ENG) and the recovery rates were calculated by comparing the measured value to the expected amount of Endoglin (ENG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-90 86
EDTA plasma(n=5) 81-93 85
heparin plasma(n=5) 97-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endoglin (ENG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endoglin (ENG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endoglin (ENG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 99-105% 98-105% 94-105%
EDTA plasma(n=5) 80-98% 91-104% 82-94% 92-101%
heparin plasma(n=5) 90-103% 86-104% 78-93% 84-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Clin Rev Allergy Immunol Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature PubMed: 26142066
Angiologia e Cirurgia Vascular Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers science:S1646706X16300052
Clinical Rheumatology Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis Pubmed:26920752
Advanced Biomedical Research Diagnostic and prognostic significance of serum soluble endoglin levels in preeclampsia and eclampsia pubmed:27563629
Annals of agricultural and environmental medicine Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines (FGF-19 and endoglin) in patients with alcoholic liver cirrhosis. pubmed:28954507
ResearchSquare The predictive role of PlGF/sENG ratio in fetal growth restriction of early beginning associated with pre-eclampsia
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA980Ra01 Recombinant Endoglin (ENG) Positive Control; Immunogen; SDS-PAGE; WB.
PAA980Ra01 Polyclonal Antibody to Endoglin (ENG) WB; IHC; ICC; IP.
LAA980Ra71 Biotin-Linked Polyclonal Antibody to Endoglin (ENG) WB; IHC; ICC.
FAA980Ra81 FITC-Linked Anti-Endoglin (ENG) Polyclonal Antibody FCM
MAA980Ra21 Monoclonal Antibody to Endoglin (ENG) WB; IHC; ICC; IP.
FAA980Ra02 Anti-Endoglin (ENG) Monoclonal Antibody FCM
FAA980Ra82 FITC-Linked Anti-Endoglin (ENG) Monoclonal Antibody FCM
FAA980Ra52 APC-Linked Anti-Endoglin (ENG) Monoclonal Antibody FCM
SEA980Ra ELISA Kit for Endoglin (ENG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA980Ra Multiplex Assay Kit for Endoglin (ENG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.