ELISA Kit for Endoglin (ENG)

CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1


This assay has high sensitivity and excellent specificity for detection of Endoglin (ENG).
No significant cross-reactivity or interference between Endoglin (ENG) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Endoglin (ENG) and the recovery rates were calculated by comparing the measured value to the expected amount of Endoglin (ENG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-90 85
EDTA plasma(n=5) 85-93 89
heparin plasma(n=5) 88-101 93


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endoglin (ENG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endoglin (ENG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endoglin (ENG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-97% 90-98% 96-103% 95-105%
EDTA plasma(n=5) 95-103% 81-92% 98-105% 85-99%
heparin plasma(n=5) 99-105% 96-105% 99-105% 88-103%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Clin Rev Allergy Immunol Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature PubMed: 26142066
Angiologia e Cirurgia Vascular Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers science:S1646706X16300052
Clinical Rheumatology Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis Pubmed:26920752
Advanced Biomedical Research Diagnostic and prognostic significance of serum soluble endoglin levels in preeclampsia and eclampsia pubmed:27563629
Annals of agricultural and environmental medicine Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines (FGF-19 and endoglin) in patients with alcoholic liver cirrhosis. pubmed:28954507
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