ELISA Kit for Caspase 8 (CASP8)

CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

Specificity

This assay has high sensitivity and excellent specificity for detection of Caspase 8 (CASP8).
No significant cross-reactivity or interference between Caspase 8 (CASP8) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 8 (CASP8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 8 (CASP8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Neurobiology Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations Pubmed:25502462
Journal of Ethnopharmacology Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells Pubmed:29555410
Clinical And Experimental Pharmacology And Physiology  Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway Pubmed:29266336
Environmental Toxicology Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice Pubmed: 31087429
Environ Toxicol Pharmacol. Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats Pubmed: 31421311
Journal of Biochemical and Molecular Toxicology Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation Pubmed: 32045101
life sciences Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats Pubmed: 32259601
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells Pubmed: 33206337
Int Immunopharmacol Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling Pubmed: 33199237
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways Pubmed: 32942000
Environ Sci Pollut Res Int Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of … Pubmed: 32915453
J Biochem Mol Toxicol Tranilast abrogates cisplatin©\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation 34047436
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA853Hu02 Recombinant Caspase 8 (CASP8) Positive Control; Immunogen; SDS-PAGE; WB.
RPA853Hu03 Recombinant Caspase 8 (CASP8) Positive Control; Immunogen; SDS-PAGE; WB.
RPA853Hu01 Recombinant Caspase 8 (CASP8) Positive Control; Immunogen; SDS-PAGE; WB.
PAA853Hu02 Polyclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC; IP.
PAA853Hu04 Polyclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC; IP.
PAA853Hu01 Polyclonal Antibody to Caspase 8 (CASP8) WB,IHC,ICC/IF
PAA853Hu03 Polyclonal Antibody to Caspase 8 (CASP8) WB,IHC,ICC/IF
FAA853Hu01 Anti-Caspase 8 (CASP8) Polyclonal Antibody Flow cytometry.
LAA853Hu81 FITC-Linked Polyclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC; IF.
LAA853Hu71 Biotin-Linked Polyclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC.
MAA853Hu21 Monoclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC; IP.
MAA853Hu23 Monoclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC; IP.
MAA853Hu25 Monoclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC; IP.
MAA853Hu24 Monoclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC; IP.
MAA853Hu29 Monoclonal Antibody to Caspase 8 (CASP8) WB
MAA853Hu27 Monoclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC; IP.
MAA853Hu22 Monoclonal Antibody to Caspase 8 (CASP8) WB; IHC; ICC; IP.
SEA853Hu ELISA Kit for Caspase 8 (CASP8) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA853Hu Multiplex Assay Kit for Caspase 8 (CASP8) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA853Hu01 ELISA Kit DIY Materials for Caspase 8 (CASP8) Main materials for "Do It (ELISA Kit) Yourself".