ELISA Kit for Prolactin (PRL)
LTH; Luteotropic Hormone
- Product No.SEA846Ov
- Organism SpeciesOvis aries; Ovine (Sheep) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range125-8,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 50pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 810
For more details, please contact local distributors! US$ 3645
For more details, please contact local distributors! US$ 6885
For more details, please contact local distributors! US$ 56700
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Prolactin (PRL).
No significant cross-reactivity or interference between Prolactin (PRL) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Prolactin (PRL) and the recovery rates were calculated by comparing the measured value to the expected amount of Prolactin (PRL) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolactin (PRL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prolactin (PRL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prolactin (PRL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|The Journal of Biological Chemistry||In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland Jcb: 39297|
|Journal of Clinical Neuroscience||TLR9 expression is associated with prognosis in patients with glioblastoma multiforme ScienceDirect: S0967586811003572|
|Sensors and Actuators B: Chemical||Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes Sciencedirect:S0925400514000720|
|Veterinary Word||Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212|
|Journal of Dairy Science||Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves publication:306322503|
|Stem Cell Research & Therapy||Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells pubmed:29096715|
|Stem Cell Research & Therapy||The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657|
|Frontiers in Molecular Neuroscience||Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355|
|Toxicology and Applied Pharmacology||The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistance Pubmed:29551354|
|Journal of Cellular Biochemistry||The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathway Pubmed:29388711|
|Catalog No.||Related products for research use of Ovis aries; Ovine (Sheep) Organism species||Applications (RESEARCH USE ONLY!)|
|SEA846Ov||ELISA Kit for Prolactin (PRL)||Enzyme-linked immunosorbent assay for Antigen Detection.|