ELISA Kit for Kidney Injury Molecule 1 (Kim1)

HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Kidney Injury Molecule 1 (Kim1).
No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Clinical Toxicology Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication PubMed: 19827907
Pediatric Nephrology KIM-1 and NGAL: new markers of obstructive nephropathy SpringerLink: v461592787w5488x
Pediatric Research Urinary Neutrophil Gelatinase-Associated Lipocalin at Birth Predicts Early Renal Function in Very Low Birth Weight Infants LWW: 10000
Archives of Medical Science Markers of kidney function in the elderly in relation to the new CKD-EPI formula for estimation of glomerular filtration rate AMS: source
Nephrology Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content PubMed: 23046363
Nanotoxicology. Comprehensive assessment of nephrotoxicity of intravenously administered sodium-oleate-coated ultra-small superparamagnetic iron oxide (USPIO) and titanium dioxide (TiO(2)) nanoparticles in rats. PubMed: 23272807
Toxicological Science A Mouse Diversity Panel Approach Reveals the Potential for Clinical Kidney Injury Due to DB289 Not Predicted by Classical Rodent Models PubMed: PMC3498743
Disease Markers Interleukin 18 as a Marker of Chronic Nephropathy in Children after Anticancer Treatment Hindawi: 369784
Journal of Biomarkers Urinary Measurement of Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Helps Diagnose Acute Pyelonephritis in a Preclinical Model Hindawi: 413853
Advances in Life Science and Technology Kidney injury molecule-1: a urinary biomarker for contrast induced acute kidney injury. Iiste: Source
Nephrology Urinary neutrophil gelatinase-associated lipocalin predicts graft loss after acute kidney injury in kidney transplant Pubmed: 24325180
Nephrology Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content. Pubmed: 23046363
BioMed Research International The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies Hindawi: 740105
Life Sci. Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model Pubmed:25476829
Diabetes &amp; Vascular Disease Research Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats Sagepub:Source
临床儿科杂志 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 Jcp-Sh:Source
Nephrology (Carlton). Tubular urinary biomarkers do not identify aetiology of acute kidney injury in kidney transplant recipients Pubmed:24666430
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion Pubmed:24816434
BMC Nephrology SYNbiotics Easing Renal failure by improving Gut microbiologY (SYNERGY): a protocol of placebo-controlled randomised cross-over trial Pubmed:24996842
Pediatric Nephrology The diagnosis of febrile urinary tract infection in children may be facilitated by urinary biomarkers Pubmed:25127917
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261
Transworld Medical Journal Kidney injury molecule-1: A urinary biomarker for contrast-induced acute kidney injury Tmjournals:Source
Int J Clin Exp Pathol Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis PubMed: 26191125
Int J Clin Exp Pathol Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice PubMed: 26045773
Mol Med Rep Vorinostat protects against calcium oxalate-induced kidney injury in mice PubMed: 26095064
Pediatr Nephrol Role of new biomarkers for predicting renal scarring in vesicoureteral reflux: NGAL, KIM-1, and L-FABP PubMed: 26324091
Journal of Pediatric Urology Urinary NGAL, KIM-1 and L-FABP concentrations in antenatal hydronephrosis PubMed: 26096437
Am J Physiol Renal Physiol Intrarenal Renin-Angiotensin System Mediates Fatty Acid-Induced ER Stress in the Kidney PubMed: 26672616
Mol Med Rep. Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via its antioxidant, anti‑inflammatory, and anti‑apoptotic activities. Pubmed:27082705
Nephrol. Dial. Transplant. Preconditioned suppression of prolyl-hydroxylases attenuates renal injury but increases mortality in septic murine models Pubmed:26908768
J Clin Lab Anal.  Kidney Injury Molecule Levels in Type 2 Diabetes Mellitus Pubmed:27132791
Med Sci Monit. Protective Effect of Dihydromyricetin Against Lipopolysaccharide-Induced Acute Kidney Injury in a Rat Model pmc:PMC4754091
Diabetes. Deficiency in Apoptosis-Inducing Factor Recapitulates Chronic Kidney Disease via Aberrant Mitochondrial Homeostasis Pubmed:26822084
Sci Rep. Once daily administration of the SGLT2 inhibitor, empagliflozin, attenuates markers of renal fibrosis without improving albuminuria in diabetic db/db mice Pubmed:27226136
Journal of Applied Life Sciences International Vitamins C and E Alleviate Nephrotoxicity-induced by Potassium Bromate in Rats Barakat812016JALSI28698.pdf
Oxidative Medicine and Cellular Longevity Synergic Effect of α-Mangostin on the Cytotoxicity of Cisplatin in a Cervical Cancer Model. pubmed:28053694
Molecular Medicine Reports Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via itsantioxidant, anti‑inflammatory, and anti‑apoptotic activities. pubmed:27082705
Journal of Agricultural and Food Chemistry Protective Effects of DHA-PC against Vancomycin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis in BALB/c Mice pubmed:29254330
Clin Exp Pharmacol Physiol. Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. pubmed:29027259
Nephron - Physiology Urinary Kidney Injury Molecule-1 but Not Urinary Neutrophil Gelatinase Associated Lipocalin Is Increased after Short Maximal Exercise 10.1159/000481179
Cellular Physiology and Biochemistry Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats … Pubmed:29550811
Frontiers in Pharmacology Exploring the therapeutic mechanism of Desmodium styracifolium on oxalate crystal-induced kidney injuries using comprehensive approaches based on … Pubmed:29950996
Molecules Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy Pubmed:29642511
American Journal of Physiology-Regulatory Integrative and Comparative Physiology Modeling heart failure risk in diabetes and kidney disease: limitations and potential applications of transverse aortic constriction in high-fat-fed mice Pubmed:29443547
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