ELISA Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2)
FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein
- Product No.SEA559Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.312-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.117ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
For more details, please contact local distributors! US$ 5814
For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 2, Intestinal (FABP2).
No significant cross-reactivity or interference between Fatty Acid Binding Protein 2, Intestinal (FABP2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Fatty Acid Binding Protein 2, Intestinal (FABP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Binding Protein 2, Intestinal (FABP2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 2, Intestinal (FABP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 2, Intestinal (FABP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Binding Protein 2, Intestinal (FABP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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|Vet Immunol Immunopathol||Immunomodulatory potential of β-glucan as supportive treatment in porcine rotavirus enteritis. pubmed:28895864|
|PLoS One||Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats pubmed:28622357|
|Journal of Immunology and Immunopathology||Effect of Porcine Rotavirus Enteritis on Concentrations of Interferonγ, Immunoglobulin-G and Intestinal Fatty Acid-Binding Protein-2 and Peripheral Leukocyte Function 10.5958/0973-9149.2017.00011.9|
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|Nutrients||Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron Pubmed:29861488|
|International Surgery Journal||Intestinal fatty acid binding protein (I-FABP) as a marker for acute intestinal ischemia Doi: 10.18203/2349-2902.isj20190058|
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|Catalog No.||Related products for research use of Chicken (Gallus) Organism species||Applications (RESEARCH USE ONLY!)|
|SEA559Ga||ELISA Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2)||Enzyme-linked immunosorbent assay for Antigen Detection.|