ELISA Kit for Immunoglobulin M (IgM)
IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region
- Product No.SEA543Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.71ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 588
For more details, please contact local distributors! US$ 2646
For more details, please contact local distributors! US$ 4998
For more details, please contact local distributors! US$ 41160
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin M (IgM).
No significant cross-reactivity or interference between Immunoglobulin M (IgM) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Immunoglobulin M (IgM) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin M (IgM) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin M (IgM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin M (IgM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin M (IgM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Poultry Science||Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 Pubmed: 24570422|
|Clin Vaccine Immunol.||Speci?c Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice Pubmed:24429068|
|Asian J Pharm Clin Res||INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS journals:3790|
|Food and Agricultural Immunology||Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers 10.108:09540105.2017.1311308|
|International Journal of Clinical and Experimental Medicine||Detection of streptococcus pyogenes antibodies in acute idiopathic urticaria ISSN:1940-5901/IJCEM0053690|
|Catalog No.||Related products for research use of Bos taurus; Bovine (Cattle) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA543Bo01||Recombinant Immunoglobulin M (IgM)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA543Bo01||Polyclonal Antibody to Immunoglobulin M (IgM)||WB; IHC; ICC; IP.|
|LAA543Bo71||Biotin-Linked Polyclonal Antibody to Immunoglobulin M (IgM)||WB; IHC; ICC.|
|MAA543Bo21||Monoclonal Antibody to Immunoglobulin M (IgM)||WB; IHC; ICC; IP.|
|SEA543Bo||ELISA Kit for Immunoglobulin M (IgM)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA543Bo01||ELISA Kit DIY Materials for Immunoglobulin M (IgM)||Main materials for "Do It (ELISA Kit) Yourself".|