ELISA Kit for Complement Component 5a (C5a)
- Product No.SEA388Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range6.25-400pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.52pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 720
For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors! US$ 6120
For more details, please contact local distributors! US$ 50400
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Complement Component 5a (C5a).
No significant cross-reactivity or interference between Complement Component 5a (C5a) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Complement Component 5a (C5a) and the recovery rates were calculated by comparing the measured value to the expected amount of Complement Component 5a (C5a) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 5a (C5a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 5a (C5a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Complement Component 5a (C5a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Molecular Immunology||Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297|
|Journal of Reproductive Immunology||Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845|
|The Journal of Clinical Investigation||Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471|
|Journal of Antimicrobial Chemotherapy||Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587|
|Journal of Orthopaedic Research||Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model Pubmed: 23070742|
|SpringerPlus||Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977|
|American Journal fo Reproductive Immunology||Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia Pubmed:25209155|
|Gynecol Obstet Invest||Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247|
|Journal of Neuroimmunology||Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358|
|International Immunopharmacology||Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602|
|Digital Repository||Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183|
|Environmental Pollution||Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia pubmed:27511439|
|Cancer Letters||Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586|
|Acta Paediatrica||Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106|
|Scientific Reports||Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis Pubmed: 30291296|
|Nutrients||The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479|
|Journal of NeuroVirology||Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976|
|PLoS One||Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004|
|Frontiers in Immunology||C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis Pubmed: 31447855|
|Phytomedicine||Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 …|
|Aging||C5aR deficiency attenuates the breast cancer development via the p38/p21 axis Pubmed: 32669478|
|STEM CELL REVIEWS AND REPORTS||A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006|
|Modulation of C5a-C5aR interactions against murine mammary cancer cell line|
|Catalog No.||Related products for research use of Canis familiaris; Canine (Dog) Organism species||Applications (RESEARCH USE ONLY!)|
|CPA388Ca21||OVA Conjugated Complement Component 5a (C5a)||Immunogen; SDS-PAGE; WB.|
|PAA388Ca08||Polyclonal Antibody to Complement Component 5a (C5a)||WB; IHC; ICC; IP.|
|SEA388Ca||ELISA Kit for Complement Component 5a (C5a)||Enzyme-linked immunosorbent assay for Antigen Detection.|