ELISA Kit for Collagen Type I Alpha 1 (COL1a1)

COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain


This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 1 (COL1a1).
No significant cross-reactivity or interference between Collagen Type I Alpha 1 (COL1a1) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Collagen Type I Alpha 1 (COL1a1) and the recovery rates were calculated by comparing the measured value to the expected amount of Collagen Type I Alpha 1 (COL1a1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-91 84
EDTA plasma(n=5) 87-95 92
heparin plasma(n=5) 86-101 96


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 1 (COL1a1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagen Type I Alpha 1 (COL1a1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-99% 97-104% 89-97% 94-102%
EDTA plasma(n=5) 89-104% 92-101% 89-98% 81-103%
heparin plasma(n=5) 98-105% 84-97% 97-105% 79-102%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology Simvastatin-loaded porous implant surfaces stimulate preosteoblasts differentiation: an in vitro study ScienceDirect: S1079210410004919
Journal of Biomedical Materials Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study Wiley: source
European Archives of Oto-Rhino-Laryngology Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats Pubmed: 24077847
J Voice. Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study Pubmed: 24495429
Arch Oral Biol. Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells. Pubmed: 24370188
Laryngoscope. Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria Pubmed:25545625
UNIVERSA MEDICINA Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture Univmed:Source
International Journal of Neuropsychopharmacology The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study. pubmed:27477355
National Natural Scientific Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396
Nephron. Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factor pubmed:28768285
Cell and Tissue Research Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and … Pubmed:29637306
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA350Mu01 Recombinant Collagen Type I Alpha 1 (COL1a1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA350Mu02 Recombinant Collagen Type I Alpha 1 (COL1a1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA350Mu01 Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) WB; IHC; ICC; IP.
PAA350Mu02 Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) WB; IHC; ICC; IP.
LAA350Mu71 Biotin-Linked Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) WB; IHC; ICC.
SEA350Mu ELISA Kit for Collagen Type I Alpha 1 (COL1a1) Enzyme-linked immunosorbent assay for Antigen Detection.