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ELISA Kit for Ubiquitin (Ub)
Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B
- Product No.SEA164Mi
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range6.25-400ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.42ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 476
For more details, please contact local distributors! US$ 680 US$ 3060 US$ 5780 US$ 47600
Specificity
This assay has high sensitivity for detection of Ubiquitin (Ub).
100% cross-reactivity of Ubiquitin (Ub) was observed among Human, Mouse, Rat, Rabbit, Canine, Porcine, Bovine, Equine.
Recovery
Matrices listed below were spiked with certain level of recombinant Ubiquitin (Ub) and the recovery rates were calculated by comparing the measured value to the expected amount of Ubiquitin (Ub) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 86-95 | 90 |
EDTA plasma(n=5) | 87-102 | 92 |
heparin plasma(n=5) | 90-102 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin (Ub) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin (Ub) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ubiquitin (Ub) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 83-93% | 92-102% | 78-88% | 79-104% |
EDTA plasma(n=5) | 98-105% | 89-96% | 78-99% | 93-103% |
heparin plasma(n=5) | 96-105% | 83-93% | 79-92% | 88-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Breast Cancer Res Treat | Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer Pubmed: 32601970 |
J Cachexia Sarcopenia Muscle | Systemic ablation of vitamin D receptor leads to skeletal muscle glycogen storage disorder in mice 34877816 |
Catalog No. | Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse) Organism species | Applications (RESEARCH USE ONLY!) |
RPA164Mi01 | Recombinant Ubiquitin (Ub) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA164Mi02 | Recombinant Ubiquitin (Ub) | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA164Mi21 | OVA Conjugated Ubiquitin (Ub) | Immunogen; SDS-PAGE; WB. |
PAA164Mi01 | Polyclonal Antibody to Ubiquitin (Ub) | WB; IHC; ICC; IP. |
PAA164Mi08 | Polyclonal Antibody to Ubiquitin (Ub) | WB; IHC; ICC; IP. |
PAA164Mi02 | Polyclonal Antibody to Ubiquitin (Ub) | WB; IHC; ICC; IP. |
MAA164Mi22 | Monoclonal Antibody to Ubiquitin (Ub) | WB; IHC; ICC; IP. |
MAA164Mi21 | Monoclonal Antibody to Ubiquitin (Ub) | WB; IHC; ICC; IP. |
MAA164Mi26 | Monoclonal Antibody to Ubiquitin (Ub) | WB; IHC; ICC; IP. |
SEA164Mi | ELISA Kit for Ubiquitin (Ub) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA164Mi | ELISA Kit for Ubiquitin (Ub) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA164Mi | Multiplex Assay Kit for Ubiquitin (Ub) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA164Mi01 | ELISA Kit DIY Materials for Ubiquitin (Ub) | Main materials for "Do It (ELISA Kit) Yourself". |
KSA164Mi11 | ELISA Kit DIY Materials for Ubiquitin (Ub) | Main materials for "Do It (ELISA Kit) Yourself". |
CGA164Mi02 | cDNA of Ubiquitin (Ub) | PCR; Protein Expression; etc. |