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ELISA Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)
CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5
- Product No.SEA116Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 29pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 279
For more details, please contact local distributors! US$ 398 US$ 1791 US$ 3383 US$ 27860
Specificity
This assay has high sensitivity and excellent specificity for detection of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES).
No significant cross-reactivity or interference between Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and the recovery rates were calculated by comparing the measured value to the expected amount of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 98-105 | 101 |
EDTA plasma(n=5) | 85-94 | 88 |
heparin plasma(n=5) | 81-98 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 81-101% | 88-96% | 80-102% | 81-97% |
EDTA plasma(n=5) | 82-95% | 83-99% | 87-94% | 85-93% |
heparin plasma(n=5) | 88-103% | 83-93% | 92-99% | 94-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
J Thorac Dis.? | Anti-endothelial cell antibodies in connective tissue diseases associated with pulmonary arterial hypertension Pubmed:Pmc4015009 |
Pharmacol Rep.? | Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression Pubmed:25150002 |
Pharmacological Reports | The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders Pubmed:25560580 |
Pharmacological Reports | Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder PubMed: 26481549 |
Veterinary Parasitology | Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140 |
Pharmacological Reports | A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity Pubmed:27208878 |
PLoS One. | Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells. pubmed:28323859 |
Experimental Parasitology | Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422 |
National Natural Scientific | Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396 |
Journal of Immunology Research | Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat Pubmed:29682587 |
Rheumatology international | Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in … Pubmed: 31468124 |
Cell Death & Disease | CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling Pubmed: 32300100 |
Medicina | Assessment of the RANTES Level Correlation and Selected Inflammatory and Pro-Angiogenic Molecules Evaluation of Their Influence on CRC Clinical Features: A … Pubmed:35208526 |
Journal of Immunology Research | Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes Pubmed:35340587 |
Reseach square | Assessment of selected interleukins (IL-6, IL-17A, IL-18, IL-23) and chemokines (RANTES, IP-10) in children with acute and chronic urticaria |