ELISA Kit for Interleukin 8 (IL8)
CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1
- Product No.SEA080Ov
- Organism SpeciesOvis aries; Ovine (Sheep) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.1pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 810
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For more details, please contact local distributors! US$ 56700
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8).
No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 8 (IL8) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Reproductive Biology and Endocrinology||Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow Rbej: 14777827|
|Acta Otolaryngol||Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa PubMed: 19958244|
|Journal of Clinical Periodontology||Experimental peri-implant mucositis at different implant surfaces. Pubmed: 24521508|
|Physiological Research||Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome. Pubmed: 2432969|
|Rheumatology International||Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186|
|J Med Life.||Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination Pubmed:Pmc4197484|
|J Clin Periodontol.||Experimental peri‐implant mucositis at different implant surfaces Pubmed:24521508|
|British Journal of Nutrition||Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers Pubmed:24846452|
|Theriogenology||Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritis Theriojournal:Source|
|Anim Reprod Sci.||Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes PubMed: 25851495|
|Медицина и здравоохранение||ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕ Article: N|
|BMC Anesthesiology||Hyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioning PubMed: 25943099|
|J Breath Res||Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery PubMed: 25719610|
|ARTICLE||The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in Egypt Profile: Mohmad_Mosaad|
|Veterinary Immunology and Immunopathology||Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period Pubmed:27090626|
|Infection, Genetics and Evolution||Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expression Pubmed:27349993|
|Asian Pacific Journal of Tropical Medicine||Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury science:S1995764516300955|
|Archives of Biological Sciences||Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol publication:299492265_Diabetes-induced_renal_failu|
|Journal of Agricultural Biotechnology||Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells 10610|
|journal of molecular and cellular cardiology||Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease. pubmed:28088561|
|Physiological Research||Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury. pubmed:28006948|
|PLOS ONE||Mechanical Ventilation Alters the Development of Staphylococcus aureus Pneumonia in Rabbit. pubmed:27391952|
|The Journal of Comenius University in Bratislava||Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injury acm.2017.16.issue-3|
|Journal of Physiology and Pharmacology||Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury. pubmed:28195073|
|Veterinary Immunology and Immunopathology||Cytokine levels in colostrum and in foals' serum pre- and post-suckling pubmed:28242000|
|Journal of Cellular Physiology||Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models pubmed:27739057|
|European Journal of Pharmacology||Cardioprotective time-window of Penehyclidine hydrochloride postconditioning: A rat study pubmed:28684235|
|PLoS One||Linezolid and atorvastatin impact on pneumonia caused by Staphyloccocus aureus in rabbits with or without mechanical ventilation pubmed:29149185|
|Archives of Oral Biology||The effect of restorative materials on cytokines in gingival crevicular fluid pubmed:28992599|
|Experimentelle periimplantäre Mukositis an unterschiedlichen Implantatoberflächen doi: 10.1111/jcpe.12240.|
|Molecular Medicine Reports||CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BB pubmed:28627614|
|ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY||Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis pubmed:28608724|
|Biological Rhythm Research||Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows 10.1080/09291016.2017.1319636|
|Scientific Reports||Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665|
|Journal of Ultrasound in Medicine||Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells. pubmed:28600899|
|Veterinary Immunology and Immunopathology||Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. pubmed:28895861|
|Biomedicine hub||Comparison of Polymyxin E and Polymyxin B as an Additive to Pulmonary Surfactant in Escherichia coli Pneumonia of Ventilated Neonatal Rabbits 10.1159/000475877|
|Anticancer Research||Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab. pubmed:28982871|
|Chronic Respiratory Disease||Inflammatory biomarkers and radiologic measurements in never-smokers with COPD: A cross-sectional study from the CODA cohort. pubmed:29117798|
|Catalog No.||Related products for research use of Ovis aries; Ovine (Sheep) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA080Ov01||Recombinant Interleukin 8 (IL8)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA080Ov01||Polyclonal Antibody to Interleukin 8 (IL8)||WB; IHC; ICC; IP.|
|LAA080Ov71||Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)||WB; IHC; ICC.|
|SEA080Ov||ELISA Kit for Interleukin 8 (IL8)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|IEA080Ov||Instant ELISA Kit for Interleukin 8 (IL8)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA080Ov01||ELISA Kit DIY Materials for Interleukin 8 (IL8)||Main materials for "Do It (ELISA Kit) Yourself".|