ELISA Kit for Interleukin 2 (IL2)
TCGF; Lymphokine; T-Cell Growth Factor; Aldesleukin
- Product No.SEA073Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.9pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
For more details, please contact local distributors! US$ 5814
For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Interleukin 2 (IL2).
No significant cross-reactivity or interference between Interleukin 2 (IL2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 2 (IL2) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 2 (IL2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 2 (IL2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 2 (IL2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 2 (IL2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Medicinal Plants Research||Evaluation of water plantain (Alisma canaliculatum A. Br. et Bouche) and mistletoe (Viscum album L.) effects on broiler growth performance, meat composition and serum biochemical parameters AcadeMic: source|
|Bone||Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration PubMed: 22796416|
|Nanomedicine||Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform PubMed: 23394132|
|Food and Chemical Toxicology||Identification and functional characterization of a novel fungal immunomodulatory protein from Postia placenta Pubmed:25662032|
|Pharmaceutical Research||Molecular Mechanisms of the Modulatory Effect of Vitamin E on Tacrolimus (FK506)-Induced Renal Injury in Rats Openview: 6Ba34F298130121B36Fc3E227Ed416F4|
|PLoS Neglected Tropical Diseases||Efficacy of Albendazole-Chitosan Microsphere-based Treatment for Alveolar Echinococcosis in Mice PubMed: 26352932|
|Int J Clin Exp Med||Berberine alleviates preeclampsia possibly by regulating the expression of interleukin-2/interleukin-10 and Bcl-2/Bax PubMed: 26629148|
|Clin Oral Investig||Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs? PubMed: 26556576|
|veterinary research forum||Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells Article_14936_6.Html:|
|Pharmaceutical Biology||Molecular mechanisms of the protective role of wheat germ oil against cyclosporin A-induced hepatotoxicity in rats PubMed: 25858514|
|Immunologic Research||Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE10 and is crucial for liver transplant tolerance PubMed: 25982596|
|J Reprod Immunol||The immunomodulating effect of seminal plasma on T cells PubMed: 25799173|
|удк||Циток Cgi-Bin: Irbis_Nbuv|
|Biomark Med||Clinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinoma PubMed: 26609634|
|Environmental Science and Pollution Research||Ameliorative effect of flaxseed oil against thiacloprid-induced toxicity in rats: hematological, biochemical, and histopathological study Pubmed:26957428|
|Advances in Experimental Medicine and Biology||Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury Pubmed:27283193|
|Research in Veterinary Science||Effects of feed supplementation with glycine chelate and iron sulfate on selected parameters of cell-mediated immune response in broiler chickens Pubmed:27473977|
|Biomedicine & Pharmacotherapy||Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate Pubmed:27261592|
|Int J Pharm Bio Sci||International Journal of Pharma and Bio Sciences ISSN cms:4987|
|Asian J Pharm Clin Res||INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS journals:3790|
|United States Patent Application||HERPES VIRUS VACCINES AND TREATMENTS y2016:0067330.html|
|BMC Veterinary Research||Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis pubmed:27553600|
|Vaccine.||Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J. pubmed:28049588|
|Taylor & Francis||Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation 09540105.2017.1306494|
|Physiological Research||Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. pubmed:28006947|
|Journal of Virology||Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed:|
|Research in Veterinary||The effect of feed supplementation with zinc chelate and zinc sulphate on selected humoral and cell-mediated immune parameters and cytokine concentration in … S0034528816303162|
|Research in Veterinary Science||Commercial gold nanocolloid inhibits synthesis of IL-2 and proliferation of porcine T lymphocytes science:article|
|Acta Vet Hung.||Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. pubmed:28244338|
|Домашня сторінка||ВПЛИВ КРОВОВТРАТИ НА ДИНАМІКУ ВМІСТУ ЦИТОКІНІВ СИРОВАТКИ КРОВІ В ПЕРІОД ГОСТРОЇ РЕАКЦІЇ НА КРАНІОСКЕЛЕТНУ ТРАВМУ 83646|
|Journal of Agricultural and Food Chemistry||Effect of boron on thymic cytokine expression, hormone secretion, antioxidant functions, cell proliferation, and apoptosis potential via the ERK1/2 signaling pathway pubmed:29032684|
|Research in Veterinary Science||The effect of feed supplementation with effective microorganisms(EM) on pro- and anti-inflammatory cytokine concentrations in pigs pubmed:28549299|
|poultry science||The effect of feed supplementation with Zakarpacki zeolite (clinoptilolite) on percentages of T and B lymphocytes and cytokine concentrations in poultry pubmed:28339915|
|Journal of Animal Physiology and Animal Nutrition||The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickens doi:10.1111|
|Molecular Medicine Reports||Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury 10.3892/mmr.2017.6837|
|Veterinary Microbiology||A bacterial ghost improves the immunological efficacy of a Newcastle disease virus inactivated vaccine pubmed:28619143|
|Canadian Journal of Gastroenterology and Hepatology||EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-ݜ傠Signaling Pathway in Rats 10.1155/2017/3057268|
|Veterinary Immunology and Immunopathology||Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. pubmed:28895861|
|Biological Rhythm Research||A study of altered cytokine rhythms associated with successful implantation in cows 10.1080/09291016.2017.1361159|
|Journal of Pain Research||Sex differences in complex regional pain syndrome type I (CRPS-I) in mice pubmed:28831269|
|Canadian Journal of Gastroenterology and Hepatology||EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-κB Signaling Pathway in Rats 10.1155/2017/3057268|
|In Vivo Study on the Hindrance Activity of Cinnamon Extract Against Aspergillus niger in Mice ISSN: 0974-4290|
|Catalog No.||Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA073Rb01||Recombinant Interleukin 2 (IL2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|CPA073Rb21||OVA Conjugated Interleukin 2 (IL2)||Immunogen; SDS-PAGE; WB.|
|PAA073Rb58||Polyclonal Antibody to Interleukin 2 (IL2)||WB; IHC; ICC; IP.|
|PAA073Rb51||Polyclonal Antibody to Interleukin 2 (IL2)||WB; IHC; ICC; IP.|
|MAA073Rb21||Monoclonal Antibody to Interleukin 2 (IL2)||WB; IHC; ICC; IP.|
|SEA073Rb||ELISA Kit for Interleukin 2 (IL2)||Enzyme-linked immunosorbent assay for Antigen Detection.|