ELISA Kit for Defensin Beta 2 (DEFb2)
B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1
- Product No.SEA072Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range62.5-4,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 26.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Defensin Beta 2 (DEFb2).
No significant cross-reactivity or interference between Defensin Beta 2 (DEFb2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Defensin Beta 2 (DEFb2) and the recovery rates were calculated by comparing the measured value to the expected amount of Defensin Beta 2 (DEFb2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 2 (DEFb2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 2 (DEFb2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Defensin Beta 2 (DEFb2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Livestock Science||The effect of Lactobacillus on the expression of porcine β-defensin-2 in the digestive tract of piglets ScienceDirect: S1871141311000126|
|Appl Environ Microbiol.||Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line Asm: Source|
|Plos Pathogens||HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in?Pseudomonas aeruginosa?Keratitis PubMed: PMC3715414|
|British Journal of Nutrition||Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese Pubmed: 24124699|
|University of Waikato||The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis Waikato:Source|
|Vaccine||On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment Pubmed:25444792|
|International Journal of Dairy Technology||Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula Doi: 10.1111|
|Molecular Nutrition Food Research||Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. Pubmed:26991948|
|Respiratory Research||Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner. pubmed:27655266|
|Key Engineering Materials||The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture. 16629795|
|Scientific Reports||Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique Pubmed:29374283|
|Frontiers in Microbiology||Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense Pubmed:29997590|
|Journal of Dermatological Science||Glyoxal-induced exacerbation of pruritus and dermatitis is associated with staphylococcus aureus colonization in the skin of a rat model of atopic dermatitis Pubmed:29496360|
|Catalog No.||Related products for research use of Sus scrofa; Porcine (Pig) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA072Po01||Recombinant Defensin Beta 2 (DEFb2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA072Po01||Polyclonal Antibody to Defensin Beta 2 (DEFb2)||WB; IHC; ICC; IP.|
|MAA072Po21||Monoclonal Antibody to Defensin Beta 2 (DEFb2)||WB; IHC; ICC; IP.|
|SEA072Po||ELISA Kit for Defensin Beta 2 (DEFb2)||Enzyme-linked immunosorbent assay for Antigen Detection.|