ELISA Kit for Interleukin 13 (IL13)
ALRH; BHR1; P600; IL-13; NC30
- Product No.SEA060Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.5pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Interleukin 13 (IL13).
No significant cross-reactivity or interference between Interleukin 13 (IL13) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 13 (IL13) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 13 (IL13) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 13 (IL13) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 13 (IL13) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 13 (IL13) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Critical Care Medicine||Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage PubMed: PMC3383056|
|Int J Clin Exp Pathol||Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation PubMed: 26722390|
|Advances in Experimental Medicine and Biology||Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury Pubmed:27283193|
|Molecular Immunology||Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-ŚĘB in murine model of asthma Pubmed:27289030|
|Journal of Agricultural Biotechnology||Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells 10610|
|BMC Veterinary Research||Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis pubmed:27553600|
|Molecular Immunology||Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma pubmed:27289030|
|Physiological Research||Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. pubmed:28006947|
|Veterinary Immunology and Immunopathology||Cytokine levels in colostrum and in foals' serum pre- and post-suckling pubmed:28242000|
|Catalog No.||Related products for research use of Bos taurus; Bovine (Cattle) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA060Bo01||Recombinant Interleukin 13 (IL13)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA060Bo01||Polyclonal Antibody to Interleukin 13 (IL13)||WB; IHC; ICC; IP.|
|MAA060Bo21||Monoclonal Antibody to Interleukin 13 (IL13)||WB; IHC; ICC; IP.|
|SEA060Bo||ELISA Kit for Interleukin 13 (IL13)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CGA060Bo01||cDNA of Interleukin 13 (IL13)||PCR; Protein Expression; etc.|