ELISA Kit for Growth Hormone (GH)
GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal
- Product No.SEA044Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.055ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 860
For more details, please contact local distributors! US$ 3870
For more details, please contact local distributors! US$ 7310
For more details, please contact local distributors! US$ 60200
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH).
No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Growth Hormone (GH) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Hormone (GH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Hormone (GH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Neuroscience Letters||The effect of peripheral administration of growth hormone on AD-like cognitive deficiency in NBM-lesioned rats PubMed: 19765635|
|Cellular Reprogramming||Changes in the Somatotrophic Axis in Genetically Identical Dogs PubMed: 21740269|
|Clinical Cancer Research||The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation status PubMed: 21642381|
|General and Comparative Endocrinology||Growth hormone expression and neuroprotective activity in a quail neural retina cell line ScienceDirect: S0016648009002457|
|Nutrition and Metabolism||Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesis Nutrition&Metabolism: source|
|Veterinary Recprd Case Report||Treatment of cervical metaphyseal fractures due to delayed ossification in an adult dog with hypopituitarism Bmj: Source|
|Journal of the American Animal Hospital Association||GH Deficiency in a Dalmatian Puppy with Megaesophagus Pubmed: 24051259|
|Biotechnology and Bioprocess Engineering||Functional expression of bovine growth hormone gene in Pleurotus eryngii Springer: Source|
|Buletin Veteriner Udayana||Profil Growth Hormone Sapi Bali Betina Pada Tiga Tipe Lahan Pemeliharaan Di Provinsi Bali Unud:Source|
|Veterinary World||Effect of prill fat supplementation on hormones, milk production and energy metabolites during mid lactation in crossbred cows Veterinaryworld:Source|
|Int J Clin Exp Med||Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831|
|Acta Histochemica||Melatonin modulates monochromatic light-induced GHRH expression in the hypothalamus and GH secretion in chicks Pubmed:26948666|
|ANNALS OF ANIMAL SCIENCE||Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87|
|Annals of Animal Science||Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87|
|Journal of Dairy Science||Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves publication:306322503|
|Jurnal Riset Akuakultur||TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA 1329|
|Trop Anim Health Prod||Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloes pubmed:28785907|
|Biological Trace Element Research||Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007|
|Revista Brasileira de Ciência Avícola||Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285|
|畜牧兽医学报||单色绿光对鸡胚褪黑激素合成的影响 DOI: 10.11843/j.issn.0366-6964.2017.05.019|
|Obesity Surgery||Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice pubmed:28853013|
|JURNAL VETERINER||Kadar Hormon Pertumbuhan Sapi Bali Lebih Rendah di Nusa Penida Daripada Daerah Bali Lainnya(LEVELS OF GROWTH HORMONE BALI CATTLE IN NUSA PENIDA LOWER THAN OTHER BALI REGIONS) 10.19087/jveteriner.2017.18.2.226|
|Veterinary World.||Factors influencing growth hormone levels of Bali cattle in Bali, Nusa Penida, and Sumbawa Islands, Indonesia. pubmed:29184372|
|Biochemistry, Genetics and Molecular Biology||Effects of Dietary Lysine Levels on the Plasma Concentrations of Growth‐Related Hormones in Late‐Stage Finishing Pigs 10.5772/intechopen.68545|
|Cellular Physiology and Biochemistry||Decreased Hepatic Lactotransferrin Induces Hepatic Steatosis in Chronic Non-Alcoholic Fatty Liver Disease Model Pubmed:29975946|
|PLoS One||Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588|
|Pakistan Journal of Zoology||Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. :|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA044Ra01||Recombinant Growth Hormone (GH)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA044Ra01||Polyclonal Antibody to Growth Hormone (GH)||WB; IHC; ICC; IP.|
|MAA044Ra21||Monoclonal Antibody to Growth Hormone (GH)||WB; IHC; ICC; IP.|
|SEA044Ra||ELISA Kit for Growth Hormone (GH)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEA044Ra||ELISA Kit for Growth Hormone (GH)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA044Ra||CLIA Kit for Growth Hormone (GH)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA044Ra01||ELISA Kit DIY Materials for Growth Hormone (GH)||Main materials for "Do It (ELISA Kit) Yourself".|