≡

CLIA Kit for Elastin Microfibril Interface Located Protein 2 (EMILIN2)

FOAP-10; Elastin Microfibril Interfacer 2

Specificity

This assay has high sensitivity and excellent specificity for detection of Elastin Microfibril Interface Located Protein 2 (EMILIN2).
No significant cross-reactivity or interference between Elastin Microfibril Interface Located Protein 2 (EMILIN2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Elastin Microfibril Interface Located Protein 2 (EMILIN2) and the recovery rates were calculated by comparing the measured value to the expected amount of Elastin Microfibril Interface Located Protein 2 (EMILIN2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-101 95
EDTA plasma(n=5) 96-105 101
heparin plasma(n=5) 94-105 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 2 (EMILIN2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastin Microfibril Interface Located Protein 2 (EMILIN2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Elastin Microfibril Interface Located Protein 2 (EMILIN2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 94-102% 97-104% 85-94%
EDTA plasma(n=5) 83-97% 83-92% 85-96% 81-99%
heparin plasma(n=5) 98-105% 83-95% 92-99% 84-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPJ161Hu01 Recombinant Elastin Microfibril Interface Located Protein 2 (EMILIN2) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ161Hu01 Polyclonal Antibody to Elastin Microfibril Interface Located Protein 2 (EMILIN2) WB; IHC; ICC; IP.
MAJ161Hu21 Monoclonal Antibody to Elastin Microfibril Interface Located Protein 2 (EMILIN2) WB; IHC; ICC; IP.
SEJ161Hu ELISA Kit for Elastin Microfibril Interface Located Protein 2 (EMILIN2) Enzyme-linked immunosorbent assay for Antigen Detection.
SCJ161Hu CLIA Kit for Elastin Microfibril Interface Located Protein 2 (EMILIN2) Chemiluminescent immunoassay for Antigen Detection.
LMJ161Hu Multiplex Assay Kit for Elastin Microfibril Interface Located Protein 2 (EMILIN2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.