CLIA Kit for Interleukin 18 Binding Protein (IL18BP)

IL18BPa; MC51L-53L-54L Homolog Gene Product; Tadekinig-alfa

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 18 Binding Protein (IL18BP).
No significant cross-reactivity or interference between Interleukin 18 Binding Protein (IL18BP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 18 Binding Protein (IL18BP) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 18 Binding Protein (IL18BP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-89 81
EDTA plasma(n=5) 83-94 87
heparin plasma(n=5) 93-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 18 Binding Protein (IL18BP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 18 Binding Protein (IL18BP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 18 Binding Protein (IL18BP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-103% 86-94% 78-105% 86-97%
EDTA plasma(n=5) 93-102% 78-97% 84-102% 78-105%
heparin plasma(n=5) 92-103% 94-102% 79-88% 78-90%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Acta Physiologica IL-18 neutralization during alveolar hypoxia improves left ventricular diastolic function in mice Pubmed:25182570
Journal of Molecular Cell Biology Role of IL‐18 in atopic asthma is determined by balance of IL‐18/IL‐18BP/IL‐18R Pubmed:28922563
Scandinavian Journal of Immunology  Altered expression of IL‐18 binding protein and IL‐18 receptor in basophils and mast cells of asthma patients Pubmed:29505668
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPH821Mu01 Recombinant Interleukin 18 Binding Protein (IL18BP) Positive Control; Immunogen; SDS-PAGE; WB.
RPH821Mu02 Recombinant Interleukin 18 Binding Protein (IL18BP) Positive Control; Immunogen; SDS-PAGE; WB.
PAH821Mu02 Polyclonal Antibody to Interleukin 18 Binding Protein (IL18BP) WB; IHC; ICC; IP.
PAH821Mu01 Polyclonal Antibody to Interleukin 18 Binding Protein (IL18BP) WB; IHC; ICC; IP.
LAH821Mu71 Biotin-Linked Polyclonal Antibody to Interleukin 18 Binding Protein (IL18BP) WB; IHC; ICC.
SEH821Mu ELISA Kit for Interleukin 18 Binding Protein (IL18BP) Enzyme-linked immunosorbent assay for Antigen Detection.
SCH821Mu CLIA Kit for Interleukin 18 Binding Protein (IL18BP) Chemiluminescent immunoassay for Antigen Detection.