CLIA Kit for Fibroblast Growth Factor 21 (FGF21)
- Product No.SCC918Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range2.74-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.49pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 21 (FGF21).
No significant cross-reactivity or interference between Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 21 (FGF21) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 21 (FGF21) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 21 (FGF21) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Substrate A||1×10mL||Substrate B||1×2mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
|Lipids in Health and Disease||Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigs Lipidworld: source|
|Endocrinology||Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression Endocrine:Source|
|Biochemical and Biophysical Research Communications||Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBH Pubmed:25449271|
|Free Radic Biol Med||Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathway PubMed: 25843656|
|Journal of diabetes and its complications||Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria. pubmed:27916484|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPC918Hu01||Recombinant Fibroblast Growth Factor 21 (FGF21)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC918Hu01||Active Fibroblast Growth Factor 21 (FGF21)||Cell culture; Activity Assays.|
|PAC918Hu01||Polyclonal Antibody to Fibroblast Growth Factor 21 (FGF21)||WB; IHC; ICC; IP.|
|FAC918Hu01||Anti-Fibroblast Growth Factor 21 (FGF21) Polyclonal Antibody||Flow cytometry.|
|MAC918Hu22||Monoclonal Antibody to Fibroblast Growth Factor 21 (FGF21)||WB; IHC; ICC; IP.|
|SEC918Hu||ELISA Kit for Fibroblast Growth Factor 21 (FGF21)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCC918Hu||CLIA Kit for Fibroblast Growth Factor 21 (FGF21)||Chemiluminescent immunoassay for Antigen Detection.|