CLIA Kit for Chemokine C-Motif Ligand 2 (XCL2)

SCYC2; SCM-1b; Small Inducible Cytokine Subfamily C Member 2; Cytokine SCM-1 beta; C motif chemokine 2; XC chemokine ligand 2

Specificity

This assay has high sensitivity and excellent specificity for detection of Chemokine C-Motif Ligand 2 (XCL2).
No significant cross-reactivity or interference between Chemokine C-Motif Ligand 2 (XCL2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Chemokine C-Motif Ligand 2 (XCL2) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine C-Motif Ligand 2 (XCL2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-104 88
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 81-92 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-Motif Ligand 2 (XCL2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-Motif Ligand 2 (XCL2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine C-Motif Ligand 2 (XCL2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-103% 89-101% 78-96% 80-101%
EDTA plasma(n=5) 94-105% 89-97% 81-98% 93-105%
heparin plasma(n=5) 84-101% 88-105% 80-101% 82-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Cancer Research The lymphotactin receptor is expressed in epithelial ovarian carcinoma and contributes to cell migration and proliferation. PubMed: 22964431
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC070Hu01 Recombinant Chemokine C-Motif Ligand 2 (XCL2) Positive Control; Immunogen; SDS-PAGE; WB.
APC070Hu01 Active Chemokine C-Motif Ligand 2 (XCL2) Cell culture; Activity Assays.
PAC070Hu01 Polyclonal Antibody to Chemokine C-Motif Ligand 2 (XCL2) WB; IHC; ICC; IP.
MAC070Hu22 Monoclonal Antibody to Chemokine C-Motif Ligand 2 (XCL2) WB; IHC; ICC; IP.
SEC070Hu ELISA Kit for Chemokine C-Motif Ligand 2 (XCL2) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC070Hu CLIA Kit for Chemokine C-Motif Ligand 2 (XCL2) Chemiluminescent immunoassay for Antigen Detection.
LMC070Hu Multiplex Assay Kit for Chemokine C-Motif Ligand 2 (XCL2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.