CLIA Kit for Apotransferrin (apoTf)
- Product No.SCB930Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range27.4-20,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 12.7pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 840
For more details, please contact local distributors! US$ 3780
For more details, please contact local distributors! US$ 7140
For more details, please contact local distributors! US$ 58800
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Apotransferrin (apoTf).
No significant cross-reactivity or interference between Apotransferrin (apoTf) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Apotransferrin (apoTf) and the recovery rates were calculated by comparing the measured value to the expected amount of Apotransferrin (apoTf) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apotransferrin (apoTf) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apotransferrin (apoTf) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apotransferrin (apoTf) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Substrate A||1×10mL||Substrate B||1×2mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
|Chest.||Heightened plasma levels of Heme Oxygenase-1 and Tissue Inhibitor of Metalloproteinase-4 as well as elevated peripheral neutrophil counts are associated with tuberculosis-diabetes comorbidity Pubmed: 24458266|
|Tuberculosis||Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity 10.1016/j.tube.2017.11.011|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|NPB930Hu01||Native Apotransferrin (apoTf)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB930Hu01||Polyclonal Antibody to Apotransferrin (apoTf)||WB; IHC; ICC; IP.|
|LAB930Hu71||Biotin-Linked Polyclonal Antibody to Apotransferrin (apoTf)||WB; IHC; ICC.|
|MAB930Hu21||Monoclonal Antibody to Apotransferrin (apoTf)||WB; IHC; ICC; IP.|
|SEB930Hu||ELISA Kit for Apotransferrin (apoTf)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCB930Hu||CLIA Kit for Apotransferrin (apoTf)||Chemiluminescent immunoassay for Antigen Detection.|
|KSB930Hu01||ELISA Kit DIY Materials for Apotransferrin (apoTf)||Main materials for "Do It (ELISA Kit) Yourself".|