CLIA Kit for Apotransferrin (apoTf)

apo-Transferrin; Apo-Tf

Specificity

This assay has high sensitivity and excellent specificity for detection of Apotransferrin (apoTf).
No significant cross-reactivity or interference between Apotransferrin (apoTf) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Apotransferrin (apoTf) and the recovery rates were calculated by comparing the measured value to the expected amount of Apotransferrin (apoTf) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 96
EDTA plasma(n=5) 96-105 101
heparin plasma(n=5) 79-93 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apotransferrin (apoTf) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apotransferrin (apoTf) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Apotransferrin (apoTf) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-93% 81-103% 82-91% 81-93%
EDTA plasma(n=5) 97-104% 94-101% 92-101% 95-102%
heparin plasma(n=5) 88-96% 84-98% 78-95% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Chest. Heightened plasma levels of Heme Oxygenase-1 and Tissue Inhibitor of Metalloproteinase-4 as well as elevated peripheral neutrophil counts are associated with tuberculosis-diabetes comorbidity Pubmed: 24458266
Tuberculosis Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity 10.1016/j.tube.2017.11.011
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
NPB930Hu01 Native Apotransferrin (apoTf) Positive Control; Immunogen; SDS-PAGE; WB.
PAB930Hu01 Polyclonal Antibody to Apotransferrin (apoTf) WB; IHC; ICC; IP.
LAB930Hu71 Biotin-Linked Polyclonal Antibody to Apotransferrin (apoTf) WB; IHC; ICC.
MAB930Hu21 Monoclonal Antibody to Apotransferrin (apoTf) WB; IHC; ICC; IP.
SEB930Hu ELISA Kit for Apotransferrin (apoTf) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB930Hu CLIA Kit for Apotransferrin (apoTf) Chemiluminescent immunoassay for Antigen Detection.
KSB930Hu01 ELISA Kit DIY Materials for Apotransferrin (apoTf) Main materials for "Do It (ELISA Kit) Yourself".