CLIA Kit for Fibroblast Growth Factor 10 (FGF10)

Keratinocyte growth factor 2

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 10 (FGF10).
No significant cross-reactivity or interference between Fibroblast Growth Factor 10 (FGF10) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 10 (FGF10) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 10 (FGF10) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 78-102 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 10 (FGF10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 10 (FGF10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 10 (FGF10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-91% 93-101% 88-95% 89-98%
EDTA plasma(n=5) 90-101% 89-102% 88-96% 85-99%
heparin plasma(n=5) 82-89% 82-96% 91-101% 90-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Inflammation Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair. pubmed:28540539
Journal of Molecular Neuroscience Dysregulation of Fibroblast Growth Factor 10 in the Peripheral Blood of Patients with Schizophrenia Pubmed: 31256336
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB882Ra01 Recombinant Fibroblast Growth Factor 10 (FGF10) Positive Control; Immunogen; SDS-PAGE; WB.
PAB882Ra01 Polyclonal Antibody to Fibroblast Growth Factor 10 (FGF10) WB; IHC; ICC; IP.
LAB882Ra81 FITC-Linked Polyclonal Antibody to Fibroblast Growth Factor 10 (FGF10) WB; IHC; ICC; IF.
LAB882Ra71 Biotin-Linked Polyclonal Antibody to Fibroblast Growth Factor 10 (FGF10) WB; IHC; ICC.
MAB882Ra21 Monoclonal Antibody to Fibroblast Growth Factor 10 (FGF10) WB; IHC; ICC; IP.
SEB882Ra ELISA Kit for Fibroblast Growth Factor 10 (FGF10) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB882Ra CLIA Kit for Fibroblast Growth Factor 10 (FGF10) Chemiluminescent immunoassay for Antigen Detection.
LMB882Ra Multiplex Assay Kit for Fibroblast Growth Factor 10 (FGF10) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.