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Polyclonal Antibody to Autophagy Related Protein 5 (ATG5)

Image (I)

DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human ATG5 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ATG5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ATG5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ATG5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human ATG5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human ATG5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human ATG5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Western Blot; Sample: U87MG cell lysate Primary Ab: 0.8µg/ml Rabbit Anti-Human ATG5 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Autophagy-Related-Protein-5-(ATG5)-PAL221Hu01.pdf

Certificate

Polyclonal Antibody to Autophagy Related Protein 5 (ATG5)

ASP; APG5; APG5L; hAPG5; Apoptosis-specific protein; Autophagy protein 5

Product No.PAL221Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPL221Hu01-Recombinant Autophagy Related Protein 5 (ATG5)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 108 252 US$ 252 US$ 360 US$ 900 US$ 3600
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ATG5. It has been selected for its ability to recognize ATG5 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine	Citations
Frontiers in Cellular Neuroscience	A Variant of the Autophagy-Related 5 Gene Is Associated with Child Cerebral Palsy. pubmed:29326554
Medical Science Monitor	Relationship of Autophagy and Apoptosis with Total Occlusion of Coronary Arteries Pubmed: 30273932
Scientific Reports	Plasma ATG5 is increased in Alzheimer's disease
Acta Neurol Belg	Association of ATG5 gene polymorphism with Parkinson's disease in a Han Chinese population 34661876

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPL221Hu01	Recombinant Autophagy Related Protein 5 (ATG5)	Positive Control; Immunogen; SDS-PAGE; WB.
PAL221Hu01	Polyclonal Antibody to Autophagy Related Protein 5 (ATG5)	WB; IHC; ICC; IP.
SEL221Hu	ELISA Kit for Autophagy Related Protein 5 (ATG5)	Enzyme-linked immunosorbent assay for Antigen Detection.
MEL221Hu	Mini Samples ELISA Kit for Autophagy Related Protein 5 (ATG5)	Enzyme-linked immunosorbent assay for Antigen Detection.
LML221Hu	Multiplex Assay Kit for Autophagy Related Protein 5 (ATG5) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.