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Mini Samples ELISA Kit for Autophagy Related Protein 5 (ATG5)
ASP; APG5; APG5L; hAPG5; Apoptosis-specific protein; Autophagy protein 5
- Product No.MEL221Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.78-50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.28ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 630
US$ 900
US$ 4050
US$ 7650
US$ 63000
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Autophagy Related Protein 5 (ATG5).
No significant cross-reactivity or interference between Mini Samples Autophagy Related Protein 5 (ATG5) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Mini Samples Autophagy Related Protein 5 (ATG5) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Autophagy Related Protein 5 (ATG5) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-104 | 86 |
| EDTA plasma(n=5) | 91-99 | 95 |
| heparin plasma(n=5) | 88-102 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Autophagy Related Protein 5 (ATG5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Autophagy Related Protein 5 (ATG5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Autophagy Related Protein 5 (ATG5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-97% | 80-92% | 97-105% | 78-89% |
| EDTA plasma(n=5) | 90-99% | 93-103% | 89-103% | 85-101% |
| heparin plasma(n=5) | 91-104% | 99-105% | 79-96% | 81-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×60µL | Assay Diluent A | 1×6mL |
| Detection Reagent B | 1×60µL | Assay Diluent B | 1×6mL |
| TMB Substrate | 1×4.5mL | Stop Solution | 1×3mL |
| Wash Buffer (30 × concentrate) | 1×10mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
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| Magazine | Citations |
| Frontiers in Cellular Neuroscience | A Variant of the Autophagy-Related 5 Gene Is Associated with Child Cerebral Palsy. pubmed:29326554 |
| Medical Science Monitor | Relationship of Autophagy and Apoptosis with Total Occlusion of Coronary Arteries Pubmed: 30273932 |
| Scientific Reports | Plasma ATG5 is increased in Alzheimer's disease |
| Acta Neurol Belg | Association of ATG5 gene polymorphism with Parkinson's disease in a Han Chinese population 34661876 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPL221Hu01 | Recombinant Autophagy Related Protein 5 (ATG5) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL221Hu01 | Polyclonal Antibody to Autophagy Related Protein 5 (ATG5) | WB; IHC; ICC; IP. |
| SEL221Hu | ELISA Kit for Autophagy Related Protein 5 (ATG5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEL221Hu | Mini Samples ELISA Kit for Autophagy Related Protein 5 (ATG5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LML221Hu | Multiplex Assay Kit for Autophagy Related Protein 5 (ATG5) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |