Packages (Simulation)

Polyclonal Antibody to Apoptosis Inducing Factor (AIF)

Image (I)

DAB staining on IHC-P;
Sample: Rat Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat AIF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Western Blot; Samples: Lane1: Rat Liver lysate; Lane2: Rat Kidney lysate; Lane3: Rat Heart lysate;
Primary Ab: 0.3µg/ml Rabbit Anti-Rat AIF Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Apoptosis-Inducing-Factor-(AIF)-PAB064Ra01.pdf

Certificate

Polyclonal Antibody to Apoptosis Inducing Factor (AIF)

PDCD8; Programmed Cell Death protein 8; Apoptosis-inducing factor 1, mitochondrial

Product No.PAB064Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB064Ra01-Recombinant Apoptosis Inducing Factor (AIF)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.59mg/ml
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 109 253 US$ 253 US$ 362 US$ 905 US$ 3620
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against AIF. It has been selected for its ability to recognize AIF in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
	РОЛЬ АПОПТОЗ ИНДУЦИРУЮЩЕГО ФАКТОРА И ОКИСЛИТЕЛЬНОГО СТРЕССА ПРИ ХРОНИЧЕСКОЙ СЕРДЕЧНОЙ НЕДОСТАТОЧНОСТИ 616-005.8
NEUROCHEMISTRY INTERNATIONAL	Redox-active DJ-1 sustains brainstem cardiovascular regulation via maintenance of mitochondrial function during mevinphos intoxication Pubmed: 32650030
Iranian Journal of Basic Medical Sciences	Chromon-3-aldehyde derivatives restore mitochondrial function in rat cerebral ischemia Pubmed: 32963739
Journal of?Pharmaceutical Sciences	Correction of mitochondrial dysfunction by 4-hydroxy-3, 5-ditretbutyl cinnamic acid in experimental Alzheimer's disease induced by A¦Â injection in rats.

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPB064Ra01	Recombinant Apoptosis Inducing Factor (AIF)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB064Ra01	Polyclonal Antibody to Apoptosis Inducing Factor (AIF)	WB; IHC; ICC; IP.
MAB064Ra21	Monoclonal Antibody to Apoptosis Inducing Factor (AIF)	WB; IHC; ICC; IP.
SEB064Ra	ELISA Kit for Apoptosis Inducing Factor (AIF)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB064Ra	Multiplex Assay Kit for Apoptosis Inducing Factor (AIF) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.