Polyclonal Antibody to Leptin (LEP)

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
European Journal of Pharmacology Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet PubMed: 20869961
Indian Journal of Pharmacology Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats Ijp: source
Journal of endocrinology Testosterone protects from metabolic syndrome-associated prostate inflammation: an experimental study in rabbit PubMed: 22010203
PLoS One. Multi-Functional Roles of Chitosan as a Potential Protective Agent against Obesity PubMed: 23342013
Molecular and Cellular Endocrinology Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs ScienceDirect: S0303720712004194
Journal of Animal and Feed Sciences Ovarian, metabolic and endocrine indexes in dairy cows with different body condition scores Ifzz: Source
British Journal of Nutrition n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigs Pubmed: 23947577
Int J Mol Sci. Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet. Pubmed: 24736781
Endocr J. CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytes Pubmed:25168658
Animal Physiology EFFECT OF LEPTIN GENE ON SERUM LEPTIN CONCENTRATION AND BEEF QUALITY IN CZECH FLECKVIEH BULLS Mendelu:Source
Evid Based Complement Alternat Med. Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645
general endocrinology Oxytocin reverse ovariectomy-induced Osteoper and body fat gain Pubmed:24506069
Respiratory Physiology & Neurobiology Maternal high-fat diet feeding during pregnancy and lactation augments lung inflammation and remodeling in the offspring Pubmed:25500158
Sci Rep Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis PubMed: 26634430
Evid Based Complement Alternat Med Tinospora crispa PubMed: 25821506
Ankara Effects of breeder age and early energy restriction on fattening performance, some meat quality traits and plasma leptin concentration of broilers Dergiler: 11
MEDICINA VETERIN Comparative measurements of the morphometric pattern and energy profile of Crioula mares in the final third of gestation, with different body condition scores Scielo: S0102-09352015000300707
Pancreatic Disorders & Therapy Pancreatic Exocrine Insufficiency Affects Not Only Digestibility of Nutrients and Growth, but Also Body Composition and Endocrinological Parameters – Study on Piglets Used As a Model for Children Journals: 2165-7092-%20S5-00
Veterinary Word Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212
Animal Anterior pituitary influence on adipokine expression and secretion by porcine adipocytes. Pubmed:27074715
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens view:87
Obes Surg. Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism. Pubmed:27185175
Acta Histochemica Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines science:S0065128116301234
J Biomed Mater Res A Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice Pubmed:26850371
Clin Epigenetics. The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model Pubmed:27110299
International Journal of Molecular Sciences Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency pubmed:27690005
Journal of Animal Science Changes in small intestinal morphometry and electrogenic nutrient transport in young pigs with pancreatic exocrine insufficiency publications:jas
International Journal of Endocrinology The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages pubmed:27990162
journal of animal science The effect of GnRH vaccination on performance, carcass, and meat quality and hormonal regulation in boars, barrows, and gilts. pubmed:27482668
journal of animal science Diet-induced modifications to milk composition have long-term effects on offspring growth in rabbits. pubmed:28380600
Evidence-Based Complementary and Alternative Medicine Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats. pubmed:28246535
Asian-Australasian Journal of Animal Sciences Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbits pubmed:27004817
Stem Cell Research & Therapy Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizingradiation. pubmed:27530249
Obesity Surgery Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in ObeseGöttingen Minipig. Effects on Glucose Metabolism. pubmed:27185175
Meat Sci Estimation of the intramuscular fat content of m. longissimus thoracis in crossbred beef cattle based on live animal measurements. pubmed:27940421
THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY Correlation of Leptin, Insulin and Glucose During Late Gestation and Early Lactation in Murrah Buffaloes 7086
Journal of Animal and Plant Sciences Effects Of Maca (Lepıdıum Meyenıı Walp) Powder On Serum Indıces And Metabolıc Responses In Racehorses 57cf073008ae83b37462323a.pdf
Biological Trace Element Research Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet article:10.1007
Revista Brasileira de Ciência Avícola Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken 10.159:1806-9061-2016-0285
Applied Animal Behaviour Science Evaluating measures of exploratory behaviour in sows around farrowing and during lactation− A pilot study 10.1016/j.jacl.2017.03.007
Proceedings of the National Academy of Sciences of the United States of America (PNAS) cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis Pubmed:29784793
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … Pubmed:29849871
Frontiers in Endocrinology Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in … Pubmed:29755407
Journal of Agricultural and Food Chemistry Metabolomics for the effect of biotin and nicotinamide on transition dairy cows Pubmed:29758980
Frontiers in Physiology Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 Pubmed:29593555
Bioscience Reports Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats Pubmed:29784871
Neurochemical Research The leptin, dopamine and serotonin receptors in hypothalamic POMC-neurons of normal and obese rodents Pubmed:29397535
PLoS One Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickens Pubmed:29373588
Biochemical and Biophysical Research Communications Chronic cold exposure results in subcutaneous adipose tissue browning and altered global metabolism in Qinghai-Tibetan plateau pika (Ochotona curzoniae) Pubmed:29626477
Alcohol and Alcoholism Leptin Expression and Gene Methylation Patterns in Alcohol-Dependent Patients with Ethyltoxic Cirrhosis—Normalization After Liver Transplantation and Implications … Pubmed:29912265
Iranian Red Crescent Medical Journal  The Effect of Aerobic Training on Tumor Growth, Adiponectin, Leptin and Ghrelin in Mice with Breast Cancer ircmj-20-03-13305.pdf
Pakistan Journal of Zoology Effects of Intrauterine Growth Retardation on Growth, Meat Quality and Muscle Fiber Composition of Pigs. :
Oncotarget Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 … Pubmed:29484120
УСПЕХИ ГЕРОНТОЛОГИИ ВЛИЯНИЕ МЕТФОРМИНА НА МЕТАБОЛИЧЕСКИЕ ПОКАЗАТЕЛИ И ГИПОТАЛАМИЧЕСКИЕ СИГНАЛЬНЫЕ СИСТЕМЫ У КРЫС С ОЖИРЕНИЕМ … AG_2018-31-01.pdf
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