Mini Samples ELISA Kit for Neurofilament, Light Polypeptide (NEFL)

CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Neurofilament, Light Polypeptide (NEFL).
No significant cross-reactivity or interference between Mini Samples Neurofilament, Light Polypeptide (NEFL) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Neurofilament, Light Polypeptide (NEFL) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Neurofilament, Light Polypeptide (NEFL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-102 98
EDTA plasma(n=5) 91-103 96
heparin plasma(n=5) 82-95 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Neurofilament, Light Polypeptide (NEFL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 81-98% 90-99% 98-105%
EDTA plasma(n=5) 91-104% 94-102% 89-97% 82-99%
heparin plasma(n=5) 91-102% 86-101% 84-91% 84-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Acta Neurologica Scandinavica Effect of carotid endarterectomy on brain damage markers Pubmed:27126899
Acta Neurologica Scandinavica Effect of carotid endarterectomy on brain damage markers. pubmed:27126899
journal of neuroimmunology Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implications Pubmed: 31254929
Cerebellum Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia 33893614
Medicine (Baltimore) Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis 34678870
J Clin Med Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients … Pubmed:35566558
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE038Hu03 Recombinant Neurofilament, Light Polypeptide (NEFL) Positive Control; Immunogen; SDS-PAGE; WB.
RPE038Hu02 Recombinant Neurofilament, Light Polypeptide (NEFL) Positive Control; Immunogen; SDS-PAGE; WB.
RPE038Hu01 Recombinant Neurofilament, Light Polypeptide (NEFL) Positive Control; Immunogen; SDS-PAGE; WB.
CPE038Hu21 OVA Conjugated Neurofilament, Light Polypeptide (NEFL) Immunogen; SDS-PAGE; WB.
PAE038Hu08 Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC; ICC; IP.
PAE038Hu02 Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC; ICC; IP.
PAE038Hu01 Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC; ICC; IP.
LAE038Hu71 Biotin-Linked Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC; ICC.
MAE038Hu25 Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC; ICC; IP.
MAE038Hu27 Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC; ICC; IP.
MAE038Hu23 Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC; ICC; IP.
MAE038Hu22 Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC; ICC; IP.
MAE038Hu21 Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC
MAE038Hu24 Monoclonal Antibody to Neurofilament, Light Polypeptide (NEFL) WB; IHC; ICC; IP.
FAE038Hu08 Anti-Neurofilament, Light Polypeptide (NEFL) Monoclonal Antibody Flow cytometry.
FAE038Hu02 Anti-Neurofilament, Light Polypeptide (NEFL) Monoclonal Antibody Flow cytometry.
FAE038Hu06 Anti-Neurofilament, Light Polypeptide (NEFL) Monoclonal Antibody Flow cytometry.
FAE038Hu04 Anti-Neurofilament, Light Polypeptide (NEFL) Monoclonal Antibody Flow cytometry.
SEE038Hu ELISA Kit for Neurofilament, Light Polypeptide (NEFL) Enzyme-linked immunosorbent assay for Antigen Detection.
HEE038Hu High Sensitive ELISA Kit for Neurofilament, Light Polypeptide (NEFL) Enzyme-linked immunosorbent assay for Antigen Detection.
MEE038Hu Mini Samples ELISA Kit for Neurofilament, Light Polypeptide (NEFL) Enzyme-linked immunosorbent assay for Antigen Detection.
CEE038Hu ELISA Kit for Neurofilament, Light Polypeptide (NEFL) Enzyme-linked immunosorbent assay for Antigen Detection.
SCE038Hu CLIA Kit for Neurofilament, Light Polypeptide (NEFL) Chemiluminescent immunoassay for Antigen Detection.
LME038Hu Multiplex Assay Kit for Neurofilament, Light Polypeptide (NEFL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSE038Hu01 ELISA Kit DIY Materials for Neurofilament, Light Polypeptide (NEFL) Main materials for "Do It (ELISA Kit) Yourself".