Multiplex Assay Kit for Selenoprotein P1, Plasma (SEPP1) ,etc. by FLIA (Flow Luminescence Immunoassay)

SEPP; SeP; SEP-P1; SELP

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Selenoprotein P1, Plasma (SEPP1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Selenoprotein P1, Plasma (SEPP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Selenoprotein P1, Plasma (SEPP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Selenoprotein P1, Plasma (SEPP1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-97 92
EDTA plasma(n=5) 78-101 92
heparin plasma(n=5) 92-105 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Selenoprotein P1, Plasma (SEPP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selenoprotein P1, Plasma (SEPP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Selenoprotein P1, Plasma (SEPP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-97% 83-104% 97-104% 93-101%
EDTA plasma(n=5) 86-101% 95-102% 79-101% 82-98%
heparin plasma(n=5) 97-104% 96-105% 78-101% 86-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SEPP1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
The Journal of Clinical Endocrinology & Metabolism Serum Selenoprotein P Levels in Patients with Type 2 Diabetes and Prediabetes: Implications for Insulin Resistance, Inflammation, and Atherosclerosis PubMed: 21677040
Diabetes & metabolism journal Increased Selenoprotein P Levels in Subjects with Visceral Obesity and Nonalcoholic Fatty Liver Disease PubMed: 23439771
Journal of Pediatric Biochemistry Trace elements, heavy metals and vitamins in Egyptian school children with iron deficiency anemia Metapress:Source
Journal of Trace Elements in Medicine and Biology Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto&#039;s thyroiditis and hypothyroidism PubMed: 26854239
OBES RES CLIN PRACT/OBESITY RESEARCH & CLINICAL PRACTICE Selenoprotein P is elevated in individuals with obesity, but is not independently associated withinsulin resistance. pubmed:27524654
Annals of Nutrition and Metabolism Acute Overfeeding Does Not Alter Liver or Adipose Tissue-Derived Cytokines in HealthyHumans. pubmed:27832637
European Journal of Nutrition DNA damage and oxidative stress response to selenium yeast in the non-smoking individuals: ashort-term supplementation trial with respect to GPX1 and SEPP1 polymorphism. pubmed:26658762
South. Clin. Ist. Euras. Evaluation of the Relationship Between Insulin Resistance and Selenoprotein P in Patients with Polycystic Ovary Syndrome DOI: 10.14744/scie.2017.87597
Arch Endocrinol Metab Serum selenium and selenoprotein-P levels in autoimmune thyroid dieases patients in a select center a transversal study DOI: 10.1590/2359-3997000000309
British Journal of Nutrition Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birth pubmed:28534447
Journal of Trace Elements in Medicine and Biology Biomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercury Pubmed:29895371
Biological & Pharmceutical Bulletin Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits Pubmed:29709922
Diabetologia Non-alcoholic fatty liver disease in the first trimester and subsequent development of gestational diabetes mellitus Pubmed: 30470912
Biological Trace Element Research Selenium Levels in Community Dwellers with Type 2 Diabetes Mellitus Pubmed: 30725267
Advances in Medical Sciences Can hepatokines be regarded as novel non-invasive serum biomarkers of intrahepatic lipid content in obese children? Pubmed: 30921653
Acute swimming exercise, but not exposure to moderate hypoxic conditions reduces circulating selenoprotein P levels in short-term, high-fat diet-fed rats Pubmed: 31236416
Hormones Selenium and selenoprotein P in nonalcoholic fatty liver disease Pubmed: 31493247
Bull Environ Contam Toxicol Soil Selenium Concentration and Residents Daily Dietary Intake in a Selenosis Area: A Preliminary Study in Yutangba Village, Enshi City, China Pubmed: 32909074
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Higher Serum Selenoprotein P Level as a Novel Inductor of Metabolic Complications in Psoriasis Pubmed: 32605214
Circulating hepassocin level in patients with stable angina is associated with fatty liver and renal function
Nat Immunol Selenium–GPX4 axis protects follicular helper T cells from ferroptosis 34413521
Reviews in Cardiovascular Medicine Selenoprotein P-1 (SEPP1) as An Early Biomarker of Acute Kidney Injury in Patients Undergoing Cardiopulmonary Bypass
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