ELISA Kit DIY Materials for Sialic Acid (SA)

  • Product No.KSS099Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range0.123-10ng/mL
  • Applicable Sensitivity0.055ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1157  US$ 1928  US$ 3470  US$ 6748  US$ 11568 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Sialic Acid (SA). No significant cross-reactivity or interference between Sialic Acid (SA) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
PLOS ONE Broad Spectrum Activity of a Lectin-Like Bacterial Serine Protease Family on Human Leukocytes Pubmed:25251283
MOLECULAR AND CELLULAR BIOCHEMISTRY Ectopic expression of 35 kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMT Pubmed: 33083950
Exp Gerontol Sialic acid associated with oxidative stress and total antioxidant capacity (TAC) expression level as a predictive indicator in moderate to severe Alzheimer's disease Pubmed: 32991981
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
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KSS099Ge11 ELISA Kit DIY Materials for Sialic Acid (SA) Main materials for "Do It (ELISA Kit) Yourself".