ELISA Kit DIY Materials for CUB And Zona Pellucida Like Domains Protein 1 (CUZD1)

ERG-1; UO-44; Transmembrane protein UO-44

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of CUB And Zona Pellucida Like Domains Protein 1 (CUZD1). No significant cross-reactivity or interference between CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
BMC Biochemistry Avoiding false discovery in biomarker research. pubmed:27474398
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