ELISA Kit DIY Materials for R-Spondin 1 (RSPO1)

RSPONDIN; Roof plate-specific spondin-1

  • Product No.KSM171Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.054ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1426  US$ 2376  US$ 4277  US$ 8316  US$ 14256 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of R-Spondin 1 (RSPO1). No significant cross-reactivity or interference between R-Spondin 1 (RSPO1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Scand J Rheumatol.? The association of radiographic progression with serum R-spondin 1 (RSPO1) levels or Dickkopf-1 (DKK1)/RSPO1 ratios in rheumatoid arthritis patients: clinical evidence for reciprocal inhibition between DKK1 and RSPO1. Pubmed:25178409
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPM171Mu01 Recombinant R-Spondin 1 (RSPO1) Positive Control; Immunogen; SDS-PAGE; WB.
RPM171Mu02 Recombinant R-Spondin 1 (RSPO1) Positive Control; Immunogen; SDS-PAGE; WB.
PAM171Mu02 Polyclonal Antibody to R-Spondin 1 (RSPO1) WB; IHC; ICC; IP.
PAM171Mu01 Polyclonal Antibody to R-Spondin 1 (RSPO1) WB; IHC; ICC; IP.
SEM171Mu ELISA Kit for R-Spondin 1 (RSPO1) Enzyme-linked immunosorbent assay for Antigen Detection.
HEM171Mu High Sensitive ELISA Kit for R-Spondin 1 (RSPO1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMM171Mu Multiplex Assay Kit for R-Spondin 1 (RSPO1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSM171Mu01 ELISA Kit DIY Materials for R-Spondin 1 (RSPO1) Main materials for "Do It (ELISA Kit) Yourself".