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ELISA Kit DIY Materials for Ethinylestradiol (EE)

Ethinyl Estradiol ; Ethynyl Estradiol; Ethinyl Oestradiol

  • Product No.KSK510Ge11
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range24.69-2000pg/mL
  • Applicable Sensitivity9.34pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1469  US$ 2448  US$ 4406  US$ 8568  US$ 14688 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Ethinylestradiol (EE). No significant cross-reactivity or interference between Ethinylestradiol (EE) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Talanta Electrochemical immunosensor for ethinylestradiol using diazonium salt grafting onto silver nanoparticles-silica–graphene oxide hybrids PubMed: 26592615
African Journal of Environmental Science and Technology Pollution by endocrine disrupting estrogens in aquatic ecosystems in Morogoro urban and peri-urban areas in Tanzania 150659
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPK510Ge11 BSA Conjugated Ethinylestradiol (EE) Immunogen; SDS-PAGE; WB.
CPK510Ge21 OVA Conjugated Ethinylestradiol (EE) Immunogen; SDS-PAGE; WB.
PAK510Ge01 Polyclonal Antibody to Ethinylestradiol (EE) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAK510Ge21 Monoclonal Antibody to Ethinylestradiol (EE) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEK510Ge ELISA Kit for Ethinylestradiol (EE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMK510Ge Multiplex Assay Kit for Ethinylestradiol (EE) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSK510Ge11 ELISA Kit DIY Materials for Ethinylestradiol (EE) Main materials for "Do It (ELISA Kit) Yourself".