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ELISA Kit DIY Materials for Survivin (Surv)

BIRC5; API4; EPR-1; Baculoviral Inhibitor Of Apoptosis Repeat Containing 5; Apoptosis Inhibitor 4; Survivin Variant 3 Alpha

  • Product No.KSC045Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1000pg/mL
  • Applicable Sensitivity5.9pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1296  US$ 2160  US$ 3888  US$ 7560  US$ 12960 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Survivin (Surv). No significant cross-reactivity or interference between Survivin (Surv) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Prion. Heligmosomoides polygyrus antigens inhibit the intrinsic pathway of apoptosis by overexpression of survivin and Bcl-2 protein in CD4 T cells Pubmed: 23787700
Medical Oncology Expression of serum survivin protein in diagnosis and prognosis of gallbladder cancer: a comparative study Pubmed:25129311
J Surg Res. Prognostic significance of survivin in resected gallbladder cancer Pubmed:25472573
Arch. Immunol. Ther. Exp. The Effects of Intestinal Nematode L4 Stage on Mouse Experimental Autoimmune Encephalomyelitis pubmed:28975357
International Journal of Nanomedicine Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor … Pubmed:29719390
Cancers (Basel) In Vivo and In Vitro Enhanced Tumoricidal Effects of Metformin, Active Vitamin D3, and 5-Fluorouracil Triple Therapy against Colon Cancer by Modulating the … Pubmed:35326689
Metformin and Calcitriol Enhance 5-Fluorouracil Tumoricidal Effects in Colon Cancer By Modulating The PI3K/Akt/PTEN/mTOR Network
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC045Mu01 Recombinant Survivin (Surv) Positive Control; Immunogen; SDS-PAGE; WB.
RPC045Mu02 Recombinant Survivin (Surv) Positive Control; Immunogen; SDS-PAGE; WB.
PAC045Mu02 Polyclonal Antibody to Survivin (Surv) WB; IHC; ICC; IP.
PAC045Mu01 Polyclonal Antibody to Survivin (Surv) WB; IHC; ICC; IP.
SEC045Mu ELISA Kit for Survivin (Surv) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC045Mu Multiplex Assay Kit for Survivin (Surv) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSC045Mu01 ELISA Kit DIY Materials for Survivin (Surv) Main materials for "Do It (ELISA Kit) Yourself".