ELISA Kit DIY Materials for Interleukin 35 (IL35)
- Product No.KSC008Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range1.56-100pg/mL
- Applicable Sensitivity
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
For more details, please contact local distributors! US$ 2100
For more details, please contact local distributors! US$ 3780
For more details, please contact local distributors! US$ 7350
For more details, please contact local distributors! US$ 12600
For more details, please contact local distributors!
The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 35 (IL35). No significant cross-reactivity or interference between Interleukin 35 (IL35) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Oncology Letters||Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. PubMed: 22783403|
|Journal of Maternal-Fetal and Neonatal Medicine||Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease Pubmed: 24175856|
|Journal of Reproductive Immunology||What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037|
|Human Immunology||The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia ScienceDirect: S0198885913005776|
|Folia Biologica (Praha)||Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Pubmed:24785110|
|Molecular Medicine Reports||Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532|
|Rom J Morphol Embryol.||The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy Pubmed:25611266|
|Journal of Immunotherapy||Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity PubMed: 25962106|
|PLOS ONE||Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis PubMed: 26204444|
|Rheumatology (Oxford)||Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease PubMed: 26231346|
|Cytokine||Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia PubMed: 26282938|
|J Biol Chem||Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway PubMed: 26085094|
|Br J Haematol||Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia PubMed: 25640666|
|Autoimmunity Reviews||New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature Pubmed:26876383|
|Clinical research||Increased plasma concentrations of interleukin 35 in patients with coronary artery disease Journal:-19|
|Clinical Rheumatology||The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis pubmed:27502600|
|Respiratory Medicine||Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis pubmed:27492538|
|International Journal of Molecular Medicine||Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines. pubmed:27460435|
|Reproductive Sciences||Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferation pubmed:28659009|
|EXPERIMENTAL AND THERAPEUTIC MEDICINE||IL‑35 may maintain homeostasis of the immune microenvironment in periodontitis issn:2542-3975|
|The Journal of Rheumatology||Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritis pubmed:29247146|
|Cellular & Molecular Immunology||Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro pubmed:28713164|
|Molecular Medicine Reports||Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. pubmed:29257310|
|Asian pacific journal of allergy and immunology||Evaluation of serum interleukin- 35 level in children with persistent asthma. pubmed:27996283|
|PLoS One||Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio pubmed:29236782|
|Hepatology Communications||Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation 10.1002/hep4.1052|
|International Journal of Clinical and Experimental Medicine||Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma ISSN:1940-5901|
|OncoTargets and Therapy||Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy. pubmed:28761357|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPC008Hu01||Recombinant Interleukin 35 (IL35)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC008Hu01||Active Interleukin 35 (IL35)||Cell culture; Activity Assays.|
|PAC008Hu01||Polyclonal Antibody to Interleukin 35 (IL35)||WB; IHC; ICC; IP.|
|MAC008Hu21||Monoclonal Antibody to Interleukin 35 (IL35)||WB; IHC; ICC; IP.|
|LAC008Hu72||Biotin-Linked Monoclonal Antibody to Interleukin 35 (IL35)||WB; IHC; ICC.|
|SEC008Hu||ELISA Kit for Interleukin 35 (IL35)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEC008Hu||High Sensitive ELISA Kit for Interleukin 35 (IL35)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PSC008Hu01||Antibody Pair for Interleukin 35 (IL35)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSC008Hu01||ELISA Kit DIY Materials for Interleukin 35 (IL35)||Main materials for "Do It (ELISA Kit) Yourself".|