ELISA Kit DIY Materials for Interleukin 35 (IL35)


The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 35 (IL35). No significant cross-reactivity or interference between Interleukin 35 (IL35) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack



Magazine Citations
Oncology Letters Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. PubMed: 22783403
Journal of Maternal-Fetal and Neonatal Medicine Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease Pubmed: 24175856
Journal of Reproductive Immunology What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037
Human Immunology The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia ScienceDirect: S0198885913005776
Folia Biologica (Praha) Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Pubmed:24785110
Molecular Medicine Reports Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532
Rom J Morphol Embryol. The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy Pubmed:25611266
Journal of Immunotherapy Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity PubMed: 25962106
PLOS ONE Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis PubMed: 26204444
Rheumatology (Oxford) Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease PubMed: 26231346
Cytokine Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia PubMed: 26282938
J Biol Chem Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway PubMed: 26085094
Br J Haematol Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia PubMed: 25640666
Autoimmunity Reviews New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature Pubmed:26876383
Clinical research Increased plasma concentrations of interleukin 35 in patients with coronary artery disease Journal:-19
Clinical Rheumatology The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis pubmed:27502600
Respiratory Medicine Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis pubmed:27492538
International Journal of Molecular Medicine Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines. pubmed:27460435
上海口腔医学 IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析 QK:98604X
Reproductive Sciences Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferation pubmed:28659009
EXPERIMENTAL AND THERAPEUTIC MEDICINE IL‑35 may maintain homeostasis of the immune microenvironment in periodontitis issn:2542-3975
The Journal of Rheumatology Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritis pubmed:29247146
Cellular & Molecular Immunology Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro pubmed:28713164
Molecular Medicine Reports Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. pubmed:29257310
Asian pacific journal of allergy and immunology Evaluation of serum interleukin- 35 level in children with persistent asthma. pubmed:27996283
PLoS One Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio pubmed:29236782
Hepatology Communications Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation 10.1002/hep4.1052
International Journal of Clinical and Experimental Medicine Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma ISSN:1940-5901
OncoTargets and Therapy Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy. pubmed:28761357
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC008Hu01 Recombinant Interleukin 35 (IL35) Positive Control; Immunogen; SDS-PAGE; WB.
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KSC008Hu01 ELISA Kit DIY Materials for Interleukin 35 (IL35) Main materials for "Do It (ELISA Kit) Yourself".