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ELISA Kit DIY Materials for Vascular Endothelial Growth Factor 121 (VEGF121)

  • Product No.KSB851Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1000pg/mL
  • Applicable Sensitivity5.7pg/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1196  US$ 1993  US$ 3587  US$ 6976  US$ 11958 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor 121 (VEGF121). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor 121 (VEGF121) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Oncogene Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069
BMC Cancer VEGF-121 plasma level as biomarker for response to anti-angiogenetic therapy in recurrent glioblastoma Pubmed:29747600
JOURNAL OF CLINICAL NEUROSCIENCE A pediatric case of anaplastic astrocytoma with a gliomatosis cerebri; the growth pattern and changes in serum VEGF-121 levels after bevacizumab treatment Pubmed: 33222957
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE Associations between VEGF isoforms and impending retinopathy of prematurity Pubmed: 32737903
international journal of oncology Activin A triggers angiogenesis via regulation of VEGFA and its overexpression is associated with poor prognosis of oral squamous cell carcinoma Pubmed: 32377747
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB851Mu01 Recombinant Vascular Endothelial Growth Factor 121 (VEGF121) Positive Control; Immunogen; SDS-PAGE; WB.
PAB851Mu01 Polyclonal Antibody to Vascular Endothelial Growth Factor 121 (VEGF121) WB; IHC; ICC; IP.
SEB851Mu ELISA Kit for Vascular Endothelial Growth Factor 121 (VEGF121) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB851Mu Mini Samples ELISA Kit for Vascular Endothelial Growth Factor 121 (VEGF121) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB851Mu Multiplex Assay Kit for Vascular Endothelial Growth Factor 121 (VEGF121) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB851Mu01 ELISA Kit DIY Materials for Vascular Endothelial Growth Factor 121 (VEGF121) Main materials for "Do It(ELISA Kit) Yourself"