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ELISA Kit DIY Materials for Jagged 1 (JAG1)

CD339; AGS; AHD; AWS; HJ1; JAGL1; Alagille Syndrome; Jagged 1 Protein

  • Product No.KSB807Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.057ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of  Jagged 1 Protein (JAG1). No significant cross-reactivity or interference between  Jagged 1 Protein (JAG1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
13 Cross-talk between the Toll-like receptor 4 and Notch1 pathways augments the inflammatory response in the interstitial cells of stenotic human aortic valves PubMed: PMC3506187
Eur J Obstet Gynecol Reprod Biol. Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosis Pubmed: 24188612
PLoS One. Augmented Osteogenic Responses in Human Aortic Valve Cells Exposed to oxLDL and TLR4 Agonist: A Mechanistic Role of Notch1 and NF-κB Interaction Pubmed:Pmc4014478
Biochim Biophys Acta.? Ligation of ICAM-1 on human aortic valve interstitial cells induces the osteogenic response: A critical role of the Notch1-NF-κB pathway in BMP-2 expression Pubmed:25101972
international journal of oral-medical sciences Jagged1 Stimulates Bone Resorption during Orthodontic Tooth Movement Dn: Jlc
Oncology letters MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer
Journal of Biological Chemistry Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328
Cell Death & Disease Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuria 33795655
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB807Hu04 Recombinant Jagged 1 (JAG1) Positive Control; Immunogen; SDS-PAGE; WB.
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RPB807Hu03 Recombinant Jagged 1 (JAG1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB807Hu01 Recombinant Jagged 1 (JAG1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB807Hu01 Polyclonal Antibody to Jagged 1 (JAG1) WB; IHC; ICC; IP.
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PAB807Hu02 Polyclonal Antibody to Jagged 1 (JAG1) WB
LAB807Hu71 Biotin-Linked Polyclonal Antibody to Jagged 1 (JAG1) WB; IHC; ICC.
LAB807Hu73 Biotin-Linked Polyclonal Antibody to Jagged 1 (JAG1) WB; IHC; ICC.
MAB807Hu22 Monoclonal Antibody to Jagged 1 (JAG1) WB; IHC; ICC; IP.
MAB807Hu21 Monoclonal Antibody to Jagged 1 (JAG1) WB
SEB807Hu ELISA Kit for Jagged 1 (JAG1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB807Hu Multiplex Assay Kit for Jagged 1 (JAG1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB807Hu01 ELISA Kit DIY Materials for Jagged 1 (JAG1) Main materials for "Do It(ELISA Kit) Yourself"