ELISA Kit DIY Materials for Neutrophil Gelatinase Associated Lipocalin (NGAL)
LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
- Product No.KSB388Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.055ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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The Abs in the kit have high sensitivity and excellent specificity for detection of Neutrophil Gelatinase Associated Lipocalin (NGAL).No significant cross-reactivity or interference between Neutrophil Gelatinase Associated Lipocalin (NGAL) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|International Journal of Nephrology and Renovascular Disease||Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancy PubMed: PMC3794962|
|International Journal of Endocrinology||Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 Pathway Pubmed: 24369464|
|International Journal of Sport Studies||Lipocalin-2: Response To Resistance Training in Obese Young Men Ijssjournal: Source|
|Asian Journal of Sports Medicine||Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young Men Tums: Source|
|European Respiratory Journal||Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlap Pubmed: 23794464|
|Acta Histochem.?||Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathy Pubmed:24802166|
|Int Urol Nephrol.||Nephrotoxic effects of varenicline as the most effective drug used for smoking cessation: a preliminary experimental study Pubmed:25812822|
|世界华人消化杂志||血清及胆汁中NGAL 对于区分良恶性胆道狭窄的临床意义 Wjgnet:Source|
|Diabetes & Vascular Disease Research||Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats Sagepub:Source|
|Biomedical & Pharmacology Journal||Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese Men Biomedpharmajournal:Source|
|Asian Journal of Physical Education and Computer Science in Sports||A Study On Thyroid Hormone And Its Effects On Exercise Ifcss:Source|
|临床儿科杂志||中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 Jcp-Sh:Source|
|Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancy Pubmed:Pmc4260273|
|Int J Chron Obstruct Pulmon Dis.||Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease Pubmed:Pmc4043430|
|Pediatric Anesthesia?||Impact of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model Pubmed:24916275|
|Inflammation.?||Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261|
|Int J Clin Exp Pathol||Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis PubMed: 26191125|
|BMC Anesthesiol||Increased cardiac index attenuates septic acute kidney injury: a prospective observational study PubMed: 25745357|
|中华临床医生杂志||中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义 Journal: Fulltext|
|PLoS One||Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats PubMed: 25909514|
|Pediatric Anesthesia||Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model Pubmed:26714833|
|Int Wound J.||From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748|
|Archives of Biological Sciences||Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol publication:299492265_Diabetes-induced_renal_failu|
|International Journal of Molecular Medicine||The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats pubmed:27430552|
|Hepatology.||Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis. pubmed:27775820|
|Lipids in Health and Disease||Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN) pubmed:28659153|
|International Wound Journal||From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748|
|Clin Exp Pharmacol Physiol.||Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. pubmed:29027259|