ELISA Kit DIY Materials for Lipocalin-2 (LCN2)

NGAL; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2


The Abs in the kit have high sensitivity and excellent specificity for detection of Neutrophil Gelatinase Associated Lipocalin (NGAL).No significant cross-reactivity or interference between Neutrophil Gelatinase Associated Lipocalin (NGAL) and analogues was observed.


1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.


Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support Pack



Magazine Citations
International Journal of Nephrology and Renovascular Disease Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancy PubMed: PMC3794962
International Journal of Endocrinology Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 Pathway Pubmed: 24369464
International Journal of Sport Studies Lipocalin-2: Response To Resistance Training in Obese Young Men Ijssjournal: Source
Asian Journal of Sports Medicine Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young Men Tums: Source
European Respiratory Journal Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlap Pubmed: 23794464
Acta Histochem.? Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathy Pubmed:24802166
Int Urol Nephrol. Nephrotoxic effects of varenicline as the most effective drug used for smoking cessation: a preliminary experimental study Pubmed:25812822
世界华人消化杂志 血清及胆汁中NGAL 对于区分良恶性胆道狭窄的临床意义 Wjgnet:Source
Diabetes & Vascular Disease Research Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats Sagepub:Source
Biomedical & Pharmacology Journal Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese Men Biomedpharmajournal:Source
Asian Journal of Physical Education and Computer Science in Sports A Study On Thyroid Hormone And Its Effects On Exercise Ifcss:Source
临床儿科杂志 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 Jcp-Sh:Source
Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancy Pubmed:Pmc4260273
Int J Chron Obstruct Pulmon Dis. Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease Pubmed:Pmc4043430
Pediatric Anesthesia? Impact of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model Pubmed:24916275
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261
Int J Clin Exp Pathol Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis PubMed: 26191125
BMC Anesthesiol Increased cardiac index attenuates septic acute kidney injury: a prospective observational study PubMed: 25745357
中华临床医生杂志 中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义 Journal: Fulltext
PLoS One Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats PubMed: 25909514
Pediatric Anesthesia Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model Pubmed:26714833
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748
Archives of Biological Sciences Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol publication:299492265_Diabetes-induced_renal_failu
International Journal of Molecular Medicine The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats pubmed:27430552
Hepatology. Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis. pubmed:27775820
Lipids in Health and Disease Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN) pubmed:28659153
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748
Clin Exp Pharmacol Physiol. Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. pubmed:29027259
Scientific Reports Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery Pubmed:29386545
Life Sciences Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity Pubmed:30036488
Molecules Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy Pubmed:29642511
American Journal of Translational Research Improved approach for normothermic machine perfusion of cold stored kidney grafts Pubmed:30018731
Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes ISBN:978-951-51-4039-5
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