ELISA Kit DIY Materials for Cyclophilin A (CYPA)
PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein
- Product No.KSA979Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range1.25-80ng/mL
- Applicable Sensitivity0.54ng/mL
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
- DownloadInstruction Manual
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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The Abs in the kit have high sensitivity and excellent specificity for detection of Cyclophilin A (CYPA).No significant cross-reactivity or interference between Cyclophilin A (CYPA) and analogues was observed.
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
|Atherosclerosis||EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation PubMed: 19758589|
|Biomarkers||Cyclophilin-A: a novel biomarker for untreated male essential hypertension Pubmed: 24164594|
|Cardiovascular Diabetology||Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease Pubmed:24502618|
|International Journal of Biological Sciences||Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction Pubmed:25552928|
|Medicine||Cyclophilin A in Ruptured Intracranial Aneurysm PubMed: 26426668|
|Mol Cell Biochem||PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease PubMed: 26702934|
|Medicine (Baltimore)||Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus PubMed: 26496315|
|International Journal of Cardiology||Cyclophilin A modulates bone marrow-derived CD117(+) cells and enhances ischemia-induced angiogenesisvia the SDF-1/CXCR4 axis. Pubmed:27057951|
|Clinica Chimica Acta||Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitor pubmed:27823952|
|Cardiovascular Diabetology||Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. pubmed:27809851|
|Clinical Science||Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity Pubmed:29382697|
|Journal of Molecular Histology||Cyclophilin a increases CD68 cell infiltration in rat experimental periodontitis Pubmed:29318411|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA979Hu01||Recombinant Cyclophilin A (CYPA)||Positive Control; Immunogen; SDS-PAGE; WB.|
|CPA979Hu21||OVA Conjugated Cyclophilin A (CYPA)||Immunogen; SDS-PAGE; WB.|
|PAA979Hu08||Polyclonal Antibody to Cyclophilin A (CYPA)||WB; IHC; ICC; IP.|
|PAA979Hu01||Polyclonal Antibody to Cyclophilin A (CYPA)||WB; IHC; ICC; IP.|
|LAA979Hu81||FITC-Linked Polyclonal Antibody to Cyclophilin A (CYPA)||WB; IHC; ICC; IF.|
|LAA979Hu71||Biotin-Linked Polyclonal Antibody to Cyclophilin A (CYPA)||WB; IHC; ICC.|
|MAA979Hu22||Monoclonal Antibody to Cyclophilin A (CYPA)||WB; IHC; ICC; IP.|
|MAA979Hu21||Monoclonal Antibody to Cyclophilin A (CYPA)||WB; IHC; ICC; IP.|
|LAA979Hu72||Biotin-Linked Monoclonal Antibody to Cyclophilin A (CYPA)||WB; IHC; ICC.|
|SEA979Hu||ELISA Kit for Cyclophilin A (CYPA)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|PSA979Hu01||Antibody Pair for Cyclophilin A (CYPA)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSA979Hu01||ELISA Kit DIY Materials for Cyclophilin A (CYPA)||Main materials for "Do It (ELISA Kit) Yourself".|