ELISA Kit DIY Materials for Clara Cell Protein 16 (CC16)

SCGB1A1; CC10; CCSP; UGB; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Clara Cell Protein 16 (CC16). No significant cross-reactivity or interference between Clara Cell Protein 16 (CC16) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Life Science Journal Pulmonary functions and blood biochemical markers for workers with and without coal worker pneumoconiosis Sciencepub: Source
Current Therapeutic Research Analysis of the Protective Biochemical and Pathologic Effects of Aminoguanidine on an Experimental Aspiration Pneumonitis Model Induced by Bile Acids PubMed: S0011393X12000562
J Thorac Dis. Effects of leflunomide on inflamation and fibrosis in bleomycine induced pulmonary fibrosis in wistar albino rats Pubmed: 24255778
J Mol Histol. The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in rats Pubmed: 24122261
Hum Exp Toxicol January The effects of α-tocopherol on oxidative damage and serum levels of Clara cell protein 16 in aspiration pneumonitis induced by bile acids Pubmed: 23023024
Journal of Thoracic Disease The evaluation of different treatment protocols for trauma-induced lung injury in rats Pubmed: 24605218
PLOS ONE Susceptibility to COPD: Differential Proteomic Profiling after Acute Smoking Pubmed:25036363
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