ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP)

  • Product No.KSA634Mu11
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range123.46-10000pg/mL
  • Applicable Sensitivity46.4pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1166  US$ 1944  US$ 3499  US$ 6804  US$ 11664 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Trypsinogen Activation Peptide (TAP). No significant cross-reactivity or interference between Trypsinogen Activation Peptide (TAP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Diabetologia Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice SpringerLink: x4l01435t34452jr
Molecular Biology Reports Prior peritoneal lavage with hot 0.9% saline induces HSP70 expression and protects against cerulein-induced acute pancreatitis in rats Pubmed: 23096089
Journal of the Pancreas Case report of mannose-binding lectin (MBL) deficiency and postoperative sepsis and coagulopathy in a patient following total pancreatectomy for chronic pancreatitis. Pubmed:25262717
Journal of Gastroenterology A small molecule inhibitor of NFκB blocks ER stress and the NLRP3 inflammasome and prevents progression of pancreatitis pubmed:27418337
Indian Journal of Pharmaceutical Sciences Effects of Calcium Channel Blockers on Trypsinogen Activation and Severity of Cerulein-induced Acute Pancreatitis in Rats IJPS:Source
Tropical Journal of Pharmaceutical Research Investigation of non-pharmaceutical cures for acute pancreatitis induced by cerulein in rats
Pancreas The Regulatory Effect of the Kinase Inhibitor PD98059 on Autophagic Flux During Trypsinogen Activation in Pancreatic Acinar Cells Pubmed: 32011537
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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CEA634Mu ELISA Kit for Trypsinogen Activation Peptide (TAP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA634Mu Multiplex Assay Kit for Trypsinogen Activation Peptide (TAP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA634Mu11 ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP) Main materials for "Do It (ELISA Kit) Yourself".